This liquid growth medium is used for preparing competent cells prior to transformation. It is a medium of choice for transformation studies. Super Optimal Broth (SOB medium) Growth Medium was first formulated by Douglas Hanahan in 1983 for preparing competent cells for transformation studies.
1. Hanahan, D., 198 3.J. Mol. Biol.166:557. 2.Sambrook J., E. F. Fritsch, and T. Maniatis. 1989.Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y