Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Sabouraud Dextrose Broth
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$36.30
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Availability: In stock
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SKU:
LQ129V
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Intended use
Recommended for cultivation of yeasts, moulds and aciduric bacteria.
Composition**
| Ingredients | g/L |
|---|---|
| Peptone, special | 10.000 |
| Dextrose (Glucose) | 20.000 |
| Final pH (at 25°C) | 5.6±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Label the ready to use LQ129V bottle. Inoculate the sample and Incubate at specified temperature and time.
Principle And Interpretation
Sabouraud Dextrose Agar is Carliers modifications (1) of the formulation described by Sabouraud (2) for the cultivation of fungi, particularly those associated with skin infections. The medium is also recommended by APHA (3). Sabouraud Dextrose Broth is also a modification by Sabouraud (4) and serves the same purpose as Sabouraud Dextrose Agar Medium 3.
Sabouraud dextrose media are peptone media supplemented with dextrose to support the growth of fungi. Peptone special provides nitrogen, vitamins, minerals, amino acids and growth factors. Dextrose (Glucose) provides an energy source for the growth of microorganisms. The low pH favors fungal growth and inhibits contaminating bacteria from clinical specimens (5). The acid reaction of the final medium is inhibitory to a large number of bacteria making it particularly useful for cultivating fungi and aciduric microorganisms. For isolation of fungi from contaminated specimens, a selective medium should be inoculated simultaneously. Incubate cultures for 4 to 6 weeks before reporting as negative.
Type of specimen
Clinical samples: skin lesions, oral lesions, oropharyngeal swabs, vaginal secretions, urine, skin and nail scrapings, urine etc.
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (6,7).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use only. For professional use only. Read the label before opening the container. Wear protective gloves/ protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Some fungi may show poor growth due to nutritional variations.
- Further isolation and biochemical tests should be carried out for confirmation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Sterile clear Sabouraud Dextrose Broth in bottle.
Colour: Light amber coloured clear solution.
Quantity of Medium: 5ml of medium in glass bottle.
pH: 5.40-5.80
Sterility Check
Passes release criteria
Growth Promotion Test
Cultural characteristics was observed after an incubation at 30-35°C for 3-5 days.
| Organism | Inoculum (CFU) |
Growth |
|---|---|---|
| Candida albicans ATCC 10231 (00054*) |
50-100 | luxuriant |
| Candida albicans ATCC 2091 (00055*) |
50-100 | luxuriant |
| #Aspergillus brasiliensis ATCC 16404 (00053*) |
50-100 | luxuriant |
| Saccharomyces cerevisiae ATCC 9763 (00058*) |
50-100 | luxuriant |
| ^Saccharomyces kudriavzevii ATCC 2601 |
50-100 | good-luxuriant |
| Trichophyton rubrum ATCC 28191 |
50-100 | luxuriant (for 5-7 days) |
Key: (*) Corresponding WDCM numbers,
(#) Formerly known as Aspergillus niger
(*) Formerly known as Saccharomyces cerevisiae
Storage and Shelf Life
On receipt store between 15-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (6,7).
| Product Name | Sabouraud Dextrose Broth |
|---|---|
| SKU | LQ129V |
| Customized Product Available | No |
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