Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Giolitti-Cantoni Broth Base, Granulated
As low as
$154.00
Availability: Out of stock
Availability: In stock
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SKU:
GM584
For selective enrichment of Staphylococcus aureus from suspected food stuffs.
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Composition
| Ingredients | Gms/Litre |
|---|---|
| Casein enzymic hydrolysate | 10.000 |
| Beef extract | 5.000 |
| Yeast extract | 5.000 |
| Mannitol | 20.000 |
| Sodium chloride | 5.000 |
| Lithium chloride | 5.000 |
| Glycine | 1.200 |
| Sodium pyruvate | 3.000 |
| Final pH (at 25°C) | 6.9±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 54.2 grams in 1000 ml distilled water. Warm gently to dissolve the medium completely. Dispense 19 ml amounts in 20mm x 200mm test tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool rapidly to room temperature and aseptically add 0.3 ml of 3.5% Potassium Tellurite Solution (FD047) to each tube. Add 0.03 ml for testing meat and meat products. Mix well before use.
Caution: Lithium chloride is harmful. Avoid bodily contact and inhalation of vapours. On contact with skin, wash with plenty of water immediately.
Principle And Interpretation
Giolitti-Cantoni Broth Base is a fluid medium employed for the recovery of low number of Staphylococci from foodstuffs as described by Giolitti and Cantoni (6). Giolitti- Cantoni Broth was also recommended by Mossel et al for detecting Staphylococcus aureus in dried milk, baby food and other food products (1). This medium was recommended as an enrichment medium by the International Dairy Federation (IDF) and APHA for detecting S.aureus in dried milk and other foods stating that the organism should be absent in 1 gram of sample (2,3). ISO committee has also recommended this medium for examination of meat and meat products (4).
Giolitti-Cantoni Broth Base contains casein enzymic hydrolysate, yeast extract and beef extract as sources of carbon, nitrogen, vitamins and minerals and B-complex vitamins. Mannitol and sodium pyruvate in the basal medium act as growth stimulants for S.aureus. Lithium chloride inhibits gram-negative lactose fermenting bacilli. Potassium tellurite and glycine inhibit gram-positive bacilli (7). Addition of sterile paraffin wax to the inoculated medium inhibits Micrococci due to creation of anaerobic conditions. Potassium tellurite concentration must be reduced as per the weight of test sample (0.1 - 0.01 gram). The medium should be inoculated as soon as it has been cooled after sterilization, otherwise absorbed oxygen should be expelled by placing the tubes in free-flowing steam for 15-20 minutes.
Inoculate 1 gram of sample or 1 ml of a suitable dilution of a sample into 19 ml of Giolitti-Cantoni Broth tubes in duplicate. Overlay the medium with 5 ml molten sterile paraffin wax and incubate at 37°C for 24-48 hours and examine daily. Blackening of the medium (usually at the bottom) within 48 hours indicates the presence of S.aureus. The blackened medium, when streaked on Baird Parker Agar (GM043/M043), shows black colonies surrounded by clear zones (5).
Quality Control
Appearance: Light yellow to brownish yellow coloured granular medium
Colour and Clarity of prepared medium: Medium amber coloured, clear solution without any precipitate
Reaction: Reaction of 5.42% w/v aqueous solution at 25°C. pH: 6.9±0.2
pH: 6.70-7.10
Cultural Response
Cultural characteristics observed with added 3.5% PotassiumTellurite Solution (FD047), after an incubation at 35-37°C for 24-48 hours.
| Organism | Inoculum (CFU) | Growth | Tellurite reduction |
|---|---|---|---|
| Staphylococcus aureus ATCC 25923 | 50-100 | good-luxuriant | positive, blackening at the bottom of the tubes or general blackening of the medium |
| Escherichia coli ATCC 25922 | >=103 | inhibited | |
| Micrococcus luteus ATCC 10240 | >=103 | inhibited | |
| Staphylococcus aureus ATCC 6538 | 50-100 | good-luxuriant | positive, blackening at the bottom of the tubes or general blackening of the medium |
| Bacillus cereus ATCC 11778 | >=103 | inhibited | |
| Pseudomonas aeruginosa ATCC 27853 | >=103 | inhibited | |
| Staphylococcus epidermidis ATCC 12228 | 50-100 | poor-fair | variable reaction |
Storage and Shelf Life
Store below 30°C in tightly closed container and prepared medium at 2-8°C. Use before expiry period on the label.
| Product Name | Giolitti-Cantoni Broth Base, Granulated |
|---|---|
| SKU | GM584 |
| Product Type | Granulated |
| Physical Form | Granular |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Mossel D. A. A., Harrewijn G. A. and Elzebroek J. M., 1973, UNICEF. 2.International Dairy Federation, 1978, IDF Standard 60A:1978, International Dairy Federation, Brussels, Belgium. 3.Marshall, (Ed.), Standard Methods for the Microbiological Examination of Dairy Products, 1993, 16th Ed., American PublicHealth Association, Washington, D.C. 4.International Organization for Standardization (ISO), 1977, Draft ISO/DIS 5551, Part 2. 5.De Waart J., Mossel D. A. A., Ten Broeke R. and Van de Moosdijk A.,1968, J. Appl. Bacteriol., 31:276. 6.Giolitti C. and Cantoni C., 1966, J. Appl. Bacteriol., 29: 395. 7.Lambin S. and German A., 1961, Precis De Microbiologie, pg. 63, Paris Masson. |
| Customized Product Available | No |
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