Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Salmonella Selective Enrichment Broth Base, Granulated
As low as
$199.10
Availability: Out of stock
Availability: In stock
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SKU:
GM1843
For selective isolation and differentiation of Salmonella species.
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Recommended for selective enrichment of Salmonella species
Recommended for selective enrichment of Salmonella species
Composition
| Ingredients | Gms / Litre |
|---|---|
| Peptone | 5.000 |
| Yeast extract | 5.000 |
| Buffer mixture | 10.000 |
| Growth mixture | 5.000 |
| Final pH (at 25°C) | 7.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 25 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add the rehydrated contents of one vial of Salmonella Selective Enrichment Supplement (FD275). Mix well and dispense as desired.
Principle And Interpretation
Salmonella are ubiquitous in the environment. These organisms are usually present in small numbers compared to coliforms; therefore it is necessary to examine a relatively large sample to isolate the organisms (1). Salmonella present in food samples may be sublethally damaged during various stages of food processing where they may be exposed to low temperatures, heat drying, radiations, various chemicals (2). These damaged cells are able to cause spoilage, and if ingested cause diseases under favourable conditions. Therefore it is important to resuscitate these damaged bacteria before enumeration.
Salmonella Enrichment Broth Base is recommended for the selective enrichment of Salmonella species within 18-24 hours.
Quality Control
Appearance: Cream to yellow coloured granular medium
Colour and clarity of prepared medium: Light yellow coloured clear solution without any precipitate
Reaction: Reaction of 2.5% w/v aqueous solution 25°C. pH: 7.0±0.2
Cultural Response: Cultural characteristics observed with added Salmonella Selective Enrichment Supplement (FD275) after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Salmonella Typhimurium ATCC 14028 | 50-100 | good-luxuriant |
| Salmonella Enteritidis ATCC 13076 | 50-100 | good-luxuriant |
| Salmonella Abony NCTC 6017 | 50-100 | good-luxuriant |
| Staphylococcus aureus ATCC 25923 | >=103 | inhibited |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Salmonella Selective Enrichment Broth Base, Granulated |
|---|---|
| SKU | GM1843 |
| Product Type | Granulated |
| Physical Form | Granular |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Cherry et al, 1972, Appl. Microbiol., 24:3342.Hartman and Minich, 1981, J. Food and Prot., 44:385 |
| Customized Product Available | No |
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