Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
Your enquiry has been submitted
Something problem with the server. Try again.
Tetrathionate Bile-Brilliant Green Broth Medium
As low as
$127.60
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MM1255
For isolation and identification of Salmonellae in accordance with Indian Pharmacopoeia.
Your enquiry has been submitted
Please login to Post an Enquiry.
Salmonellae#CC293D
Intended Use
Tetrathionate Bile Brilliant Green Broth is used for isolation and identification of Salmonellae in accordance with Indian Pharmacopoeia.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Peptone | 8.600 |
| Dehydrated Oxbile | 8.000 |
| Sodium chloride | 6.400 |
| Calcium carbonate | 20.000 |
| Potassium tetrathionate | 20.000 |
| Brilliant green | 0.070 |
| pH after heating (at 25°C) | 7.0±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 63.07 grams in 1000 ml purified/distilled water. Heat just to boiling. DO NOT AUTOCLAVE OR REHEAT. Dispense as desired.
Due to presence of Calcium Carbonate, the prepared medium forms opalescent solution with white precipitate
Principle And Interpretation
Tetrathionate Bile Brilliant Green Broth is prepared as per the recommendation of Indian Pharmacopoeia (1) for microbial limit tests and isolation-identification of Salmonella species from pharmaceutical, foods, water and other materials of sanitary importance.
Peptone provides nitrogenous nutrients to the Salmonellae. Brilliant green and ox-bile inhibit both gram-positive as well as some selected gram-negative organisms while it promotes the growth of Salmonella. Potassium tetrathionate inhibits normal flora of faecal specimens. Sodium chloride helps in maintaining osmotic equilibrium. Calcium carbonate neutralizes the acids produced by reduction of tetrathionate.
After incubation streak onto differential medium for isolation and identification. Medium is not suitable for growth of Salmonella Typhi and Salmonella Paratyphi (2). Proteus may proliferate in the medium, which can make the medium less effective.
Quality Control
Appearance: Light yellow to greenish yellow homogeneous free flowing powder
Colour and Clarity of prepared medium: Bluish green opalescent solution with white precipitate
Reaction: Reaction of 6.3% w/v aqueous solution after heating. pH : 7.0±0.2
pH: 6.80-7.20
Cultural Response
Cultural characteristics observed after enrichment in Broth Medium I at 36-38°C for 48 hours, and then subcultured on Xylose Lysine Deoxycholate Agar (MM031) and incubated at 36-38°C for 24-48 hours.
| Organism | Inoculum (CFU) | Growth | Observed Lot Recovery value (CFU) | Colour of Colony | Incubation temperature |
|---|---|---|---|---|---|
| Salmonella Typhimurium ATCC 14028 | 50-100 | luxuriant | 25-100 >=50% | red with black centres | 18-24 hrs |
| Salmonella Abony NCTC 6017 | 50-100 | good-luxuriant | 25-100 >=50% | red with black centres | 18-24 hrs |
| Salmonella Typhi ATCC 6539 | 50-100 | luxuriant | 25-100 >=50% | Red | 18-24 hrs |
| Salmonella Enteritidis ATCC 13076 | 50-100 | luxuriant | 25-100 >=50% | red with black centres | 18-24 hrs |
| Staphylococcus aureus ATCC 6538 | >=10³ | inhibited | 0 0% | >=24 hrs | |
| Escherichia coli ATCC 8739 | 50-100 | fair | 10-30 20-30% | yellow | 18-24 hrs |
| Escherichia coli NCTC 9002 | 50-100 | fair | 10-30 20-30% | yellow | 18-24 hrs |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Tetrathionate Bile-Brilliant Green Broth Medium |
|---|---|
| SKU | MM1255 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Indian Pharmacopoeia, 2007, Vol. II, Published by the Controller of Publications, New Delhi, Government of India, Ministryof Health and Family Welfare. |
| Customized Product Available | No |
Write Your Own Review
Tetrathionate Brilliant Green HiVeg™ Broth
As low as
$116.60
Tetrathionate Brilliant Green Bile Broth, Granulated
As low as
$127.60
Tetrathionate Brilliant Green Bile Broth
As low as
$116.60
Broth Medium I (Tetrathionate Bile-Brilliant Green Broth)
As low as
$127.60
Broth Medium I (Tetrathionate Bile-Brilliant Green Broth)
As low as
$127.60
Tetrathionate Brilliant Green HiCynth™ Broth
As low as
$127.60
Upsell Products
Tetrathionate Broth
As low as
$51.70