Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Broth Medium I (Tetrathionate Bile-Brilliant Green Broth)
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$127.60
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SKU:
ME1255
For isolation and identification of Salmonellae in accordance with European Pharmacopoeia.
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Intended Use
Recommended for isolation and identification of Salmonella in accordance with EP.
Composition
| Ingredients | g/L |
|---|---|
| Peptone | 8.600 |
| Bile, dried # | 8.000 |
| Sodium chloride | 6.400 |
| Calcium carbonate | 20.000 |
| Potassium tetrathionate | 20.000 |
| Brilliant green | 0.070 |
| pH after heating | 7.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Ox-bile dried
Directions
Suspend 63.07 grams in 1000 ml purified/distilled water. Heat just to boiling. DO NOT AUTOCLAVE OR REHEAT. Dispense into tubes or flasks as desired.
Note: Due to presence of Calcium Carbonate, the prepared medium forms opalescent solution with white precipitate.
Principle And Interpretation
Tetrathionate Brilliant Green Bile Broth cited as Broth Medium I and is formulated as per the recommendation of European Pharmacopoeia (1) It is recommended for isolation and identification of Salmonella species in the tests prescribed for sterility checking in the Pharmacopoeia. It is employed to detect Salmonella from pharmaceutical, foods, water and other materials of sanitary importance.
Peptone provides nitrogenous nutrients to the Salmonellae. Brilliant green and Bile, dried inhibit both gram-positive as well as some selected gram-negative organisms. Potassium tetrathionate inhibits normal flora of faecal specimens. Sodium chloride helps in maintaining osmotic equilibrium. Calcium carbonate neutralizes the acids produced by reduction of tetrathionate.
Medium is not suitable for growth of Salmonella Typhi and Salmonella Paratyphi (2). After incubation streak onto two of any of the selective like Agar Medium J, Agar Medium K, or Agar Medium L. Incubate at 35-37°C for 18-72 hours and observe for typical colony appearance and characteristics (1).
Type of specimen
Pharmaceutical samples
Specimen Collection and Handling
Follow appropriate techniques for handling specimens as per established guidelines (1). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- For further confirmation, streak the enriched cultures after incubation, on plates of Brilliant Green Agar (M016), MacConkey Agar (M081) and Bismuth Sulphite Agar (M027).
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Light yellow to greenish yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Bluish green opalescent solution with white precipitate
pH
6.80-7.20
Cultural Response
Cultural characteristics observed after enrichment in Broth Medium I at 41-43°C for 18-24 hours, and then subcultured on Agar Medium K (Xylose Lysine Deoxycholate Agar),(ME031) and Agar Medium L (Brilliant Green, Phenol red, lactose monohydrate Sucrose Agar), (ME016) and incubated at 35-37°C for 18-72 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of Colony | Incubation period |
|---|---|---|---|---|---|
| Growth on Agar Medium K | |||||
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | luxuriant | >=50% | red with black centres | 18-72 hrs |
| Salmonella Abony NCTC 6017 (00029*) | 50-100 | good-luxuriant | >=50% | red with black centres | 18-72 hrs |
| Additional Microbiological Testing Growth on Agar Medium K | |||||
| Salmonella Enteritidis ATCC 13076 (00030*) | 50-100 | luxuriant | >=50% | red with black centres | 18-72 hrs |
| Staphylococcus aureus subsp. aureus ATCC 6538 (00032*) | >=103 | inhibited | 0% | >=72 hrs | |
| Escherichia coli ATCC 8739 (00012*) | 50-100 | fair | 20-30% | yellow | 18-72 hrs |
| Growth on Agar Medium L | |||||
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | luxuriant | >=50% | pinkish white | 18-24 hrs |
| Salmonella Abony NCTC 6017 (00029*) | 50-100 | luxuriant | >=50% | pinkish white | 18-24 hrs |
| Additional Microbiological Testing Growth on Agar Medium L | |||||
| Salmonella Enteritidis ATCC 13076 (00030*) | 50-100 | luxuriant | >=50% | pinkish white | 18-24 hrs |
| Staphylococcus aureus subsp. aureus ATCC 6538 (00032*) | >=103 | inhibited | 0% | >=24 hrs | |
| Escherichia coli ATCC 8739 (00012*) | 50-100 | fair | 20-30% | yellow | 18-24 hrs |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
References
- European Pharmacopoeia, 2022, 10 th volume, European Directorate for the quality of medicines & Healthcare.
- MacFaddin J.F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. I, Williams and Wilkins, Baltimore.
- Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.
- Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1.
| Product Name | Broth Medium I (Tetrathionate Bile-Brilliant Green Broth) |
|---|---|
| SKU | ME1255 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Mueller L., 1923, C. R. Soc. Biol., (Paris), 89, 434. |
| Customized Product Available | No |
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