Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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HiEncap™ SOB Growth Medium
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$0.00
Availability: Out of stock
Availability: In stock
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SKU:
ECG014CCL
For preparing competent host cells prior to transformation.
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Composition
| Ingredients | Gms / Litre |
|---|---|
| Tryptone | 20.000 |
| Yeast extract | 5.000 |
| Sodium chloride | 0.500 |
| Magnesium sulphate, hepta hydrate | 5.000 |
Formula adjusted, standardized to suit performance parameters
Directions
Each capsule contains 6.99 gms of medium. Suspend 1 capsule in 250 ml (4 capsules in 1000 ml) distilled or purified water.
Heat to boiling to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 15 lbs pressure (121° C) for 15 minutes.
Principle And Interpretation
SOB Growth Medium is used for preparing competent cells prior to transformation. Super Optimal Broth (SOB medium) Growth Medium was first formulated by Douglas Hanahan in 1983 as a nutritionally enriched growth media for bacteria, especially Escherichia coli (1). It is used in the preparation and transformation of chemically competent cells. E. coli cells are first made competent during transformation where perforations are made in the bacterial cells so that the foreign DNA can penetrate the cells (2). To endure this process, competent cells require a rich isotonic medium. Tryptone provides nitrogen, amino acids and other growth factors which permit the cells to go through the stress of transformation. Vitamins and trace elements are contained in Yeast Extract. Sodium chloride provides essential ions for transport and osmotic balance. Magnesium sulfate provides magnesium ions which are required in a variety of enzymatic reactions, including DNA replication (2).
Type of specimen
Not applicable, Recombinant Escherichia coli culture under study.
Specimen Collection and Handling
Follow appropriate laboratory techniques for handling culture, processing as per guidelines and local standards (2). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- If the cells are not grown to desired competency required in the medium, transformation may not be successful.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Gelatin capsule containing light yellow coloured granular media
Prepared Medium
Light amber coloured, clear solution without any precipitate
Quantity
Each capsule contains 6.99 grams of medium sufficient for 250 ml media
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Escherichia coli ATCC 23724 | 50-100 | good-luxuriant |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | good-luxuriant |
| Escherichia coli DH5 alpha MTCC 1652 | 50-100 | good-luxuriant |
Key = * Corresponding WDCM numbers
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with with current laboratory techniques (3,4).
| Product Name | HiEncap™ SOB Growth Medium |
|---|---|
| SKU | ECG014CCL |
| Product Type | HiEncap™ |
| Physical Form | Granulated Media in Gelatin Capsule |
| Origin | Animal |
| Packaging type | HDPE Jar |
| References | 1. Hanahan, D., 198 3.J. Mol. Biol.166:55 2.Sambrook J., E. F. Fritsch, and T. Maniatis. 198 |
| Customized Product Available | No |
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HiEncap™ SOB Growth Medium
As low as
$0.00
SOB Growth Medium
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