Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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HiEncap™ Potato Dextrose Agar
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Availability: In stock
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SKU:
EC096D
For isolation and enumeration of yeasts and moulds.
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Intended Use
Recommended for isolation and enumeration of yeasts and moulds.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Potatoes, infusion from | 200.000 |
| Dextrose (Glucose) | 20.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 5.6±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Each capsule contains 19.5 grams of medium. Suspend 1 capsule in 500 ml (2 capsules in 1000 ml) distilled or purified water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well before dispensing. In specific work, when pH 3.5 is required, acidify the medium with sterile 10% tartaric acid. The amount of acid required for 100 ml. of sterile, cooled medium is approximately 1 ml. Do not heat the medium after addition of the acid.
Principle And Interpretation
Potato Dextrose Agar is recommended by APHA (9) and F.D.A. (4) for plate counts of yeasts and moulds in the examination of foods and dairy products (11). Potato Dextrose Agar is also used for stimulating sporulation, for maintaining stock cultures of certain dermatophytes and for differentiation of typical varieties of dermatophytes on the basis of pigment production (8). It is also recommended by USP (10), BP (2), EP (3) and JP (6) for growth of fungi. Potato infusion and dextrose promote luxuriant fungal growth. Adjusting the pH of the medium by tartaric acid to 3.5, inhibits the bacterial growth. Heating the medium after acidification should be avoided as it may hydrolyse the agar which can render the agar unable to solidify.
Type of specimen
Food and dairy samples; Water samples
Specimen Collection and Handling
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (4,9,11). For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards.(1) After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets
Limitations
- Heating the medium after acidification should be avoided as it may hydrolyse the agar which can render the agar unable to solidify.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Gelatin capsule containing cream to yellow coloured granular media
Gelling: Firm, comparable with 1.5% Agar gel
Colour and clarity of prepared medium
Light amber coloured clear to slightly opalescent gel forms in Petri plates
Quantity
Each capsule contains 19.5 grams of medium sufficient for 500 ml media
Reaction
pH of 3.9% w/v aqueous solution at 25°C. pH : 5.6±0.2
pH
5.40-5.80
Cultural Response
Cultural characteristics observed after incubation at 20-25 °C for 2-5 days.
| Organism | Inoculum (CFU) | Growth | Recovery | Incubation temperature | Incubation period |
|---|---|---|---|---|---|
| Candida albicans ATCC 10231 (00054*) | 50-100 | luxuriant | >=70% | 20-25 °C | 2-3 d |
| #Aspergillus brasiliensis ATCC 16404 (00053*) | 50-100 | luxuriant | >=70% | 20-25 °C | 5-7 d |
| Saccharomyces cerevisiae ATCC 9763 (00058*) | 50-100 | luxuriant | >=70% | 30-35 °C | 2-5 d |
| Rhodotorula mucilaginosa DSM 70403 | luxuriant | 20-25 °C | 3-5 d | ||
| Geotrichum candidum DSM 1240 | good- luxuriant | 25-30 °C | 3-5 d | ||
| Penicillium commипае ATCC 10248 | fair -good | 25 -30 °C | 3-5 d | ||
| Trichophyton ajelloi ATCC 28454 | fair-good | 25-30 °C | 3-7 d |
Key
* Corresponding WDCM numbers # Formerly known as Aspergillus niger
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,7).
| Product Name | HiEncap™ Potato Dextrose Agar |
|---|---|
| SKU | EC096D |
| Application/ Industry | Food, Dairy |
| Product Type | HiEncap™ |
| Physical Form | Granulated Media in Gelatin Capsule |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE Jar |
| References | 1.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., APHA, Washington, D.C. 2.FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, DC. 3.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed., APHA Inc., Washington, D.C. 4.MacFaddin J. F., 1985, Media for the Isolation-Cultivation Identification-Maintenance of Medical Bacteria, Vol.1, Williamsand Wilkins, Baltimore5.The United States Pharmacopoeia, 2009, The United States Pharmacopoeial Convention. Rockville, MD. 6.British Pharmacopoeia, 2009, The Stationery office British Pharmacopoeia7.European Pharmacopoeia, 2009, European Dept. for the quality of Medicines. 8.Japanese Pharmacopoeia, 2008 |
| Customized Product Available | No |
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