Fetal Bovine Serum Mesenchymal Stem Cell Tested Australia origin, Sterile filtered

Fetal bovine serum (FBS) is a ubiquitously used essential supplement in cell culture media. FBS is a cocktail of proteins, vitamins, carbohydrates, lipids, hormones, growth factors, minerals and trace elements and is used as an universal growth supplement effective for most types of human and animal (including insect) cells. The major functions of serum in culture media are to provide: i. Hormonal factors stimulating cell growth and proliferation and promoting differentiated functions. ii. Transport proteins carrying hormones (e.g.transcortin) minerals and trace elements and lipids (e.g. lipoproteins) iii. Attachment and spreading factors, acting as germination points for cell attachment. iv. Stabilizing and detoxifying factors needed to maintain pH or to inhibit proteases either directly, such as αantitrypsin or α2-macroglobulin, or indirectly, by acting as an unspecific sink for proteases and other (toxic) molecules. MSC qualified sera are suitable for culturing mesenchymal stem cells (MSC) which are undifferentiated cells that have the ability to differentiate into multiple lineages. A major challenge in culturing MSC is the difficulty to maintain them in undifferentiated state while avoiding their differentiation into unwanted lineage. Serum contains many unknown factors, nutrient and molecules in variable concentration that may lead to unwanted differentiation of stem cells. MSC qualified sera are prescreened for clonal efficiency, expansion and differentiation potential of mesenchymal stem cells into adipogenic, osteogenic and chondrogenic lineages. RM10846 is collected from AQIS (Australian Quarantine and Inspection Service) approved abattoirs in Australia. Australia is considered to be free of BSE and FMD and belongs to GBR 1 category.