Tetracycline Negative, Sterile Filtered

Fetal bovine serum (FBS) is a ubiquitously used essential supplement in cell culture media. FBS is a cocktail of proteins, vitamins, carbohydrates, lipids, hormones, growth factors, minerals and trace elements and is used as an universal growth supplement effective for most types of human and animal (including insect) cells. The major functions of serum in culture media are to provide: i. Hormonal factors stimulating cell growth and proliferation and promoting differentiated functions. ii. Transport proteins carrying hormones (e.g. transcortin), minerals and trace elements (e.g. transferrin) and lipids (e.g. lipoproteins) iii. Attachment and spreading factors, acting as germination points for cell attachment. iv. Stabilizing and detoxifying factors needed to maintain pH or to inhibit proteases either directly, such as αantitrypsin or α2-macroglobulin, or indirectly, by acting as an unspecific sink for proteases and other (toxic) molecules. RM10686 is sourced in countries approved for import into the European Union by European Commission. Currently this includes Central and South America, USA, Canada, Australia, New Zealand and South Africa. This serum is collected and processed in facilities registered and inspected by the competent authority in the country of origin. EU approved serum can be freely moved between EU member countries and many other countries outside of Europe where the USDA or FDA regulations are not required. Tetracycline (Tet) tested serum is specifically qualified for use in Tet-inducible gene expression systems. The Tet-On and Tet-Off expression systems use a tetracycline-controlled transactivator which interacts with a promoter to regulate expression of the gene of interest. Fetal bovine serum often contains the antibiotic Tet and extraneous Tet results in unintended expression leading to erroneous results. Tet test is performed using liquid chromatography electrospray ionization tandem mass spectrometry method. Tet negative serum contains less than 0.05µg/ml tetracycline.