Dialyzed, Sterile Filtered

Fetal bovine serum (FBS) is a ubiquitously used essential supplement in cell culture media. FBS is a cocktail of proteins, vitamins, carbohydrates, lipids, hormones, growth factors, minerals and trace elements and is used as an universal growth supplement effective for most types of human and animal (including insect) cells. The major functions of serum in culture media are to provide: i. Hormonal factors stimulating cell growth and proliferation and promoting differentiated functions. ii. Transport proteins carrying hormones (e.g. transcortin), minerals and trace elements (e.g. transferrin) and lipids (e.g. lipoproteins) iii. Attachment and spreading factors, acting as germination points for cell attachment. iv. Stabilizing and detoxifying factors needed to maintain pH or to inhibit proteases either directly, such as αantitrypsin or α2-macroglobulin, or indirectly, by acting as an unspecific sink for proteases and other (toxic) molecules. RM10685 is dialyzed fetal bovine serum useful in cell culture systems requiring a defined environment. The process of dialysis results in depleting the serum of low molecular weight molecules (less than 10000 MW) such as nucleotides, amino acids, hormones, cytokines and salt. The serum is dialyzed against a solution of sterile PBS using a 10000Dalton cutoff membrane. This serum would be ideal for non radioactive and radioactive incorporation studies.