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Blood Agar Base No. 2, HiVeg™

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MV834
After addition of blood, medium permits maximum recovery of Streptococci, Pneumococci and other fastidious pathogenic microorganisms without interfering with their haemolytic reactions.

Product Overview

Blood Agar Base No. 2, HiVeg™ / with 1.2% Agar, HiVeg™ is specially devised to permit the maximum recovery of fastidious pathogenic microorganisms without interfering with their haemolytic reactions.

Composition

Ingredients MV834 Grams/Litre MV834A Grams/Litre
HiVeg peptone No.3 15.00 15.00
HiVeg extract No.2 2.50 2.50
Yeast extract 5.00 5.00
Sodium chloride 5.00 5.00
Agar 15.00 12.00
Final pH (at 25°C) 7.4± 0.2

Formula adjusted, standardized to suit performance parameters.

Product Profile

Vegetable based (Code MV)

  • MV834/MV834A
  • HiVeg peptone No. 3
  • HiVeg extract No. 2

Animal based (Code M)

  • M834/M834A
  • Proteose peptone
  • Liver extract

Recommended for

Maximum recovery of fastidious pathogenic microorganisms without interfering with their haemolytic reactions.

Reconstitution

  • (MV834): 42.5 g/l
  • (MV834A): 39.5 g/l

Quantity on preparation

  • (500g): (MV834): 11.76 L
  • (100g): (MV834): 2.35 L
  • (500g): (MV834A): 12.65 L

pH (25°C)

7.4 ± 0.2

Supplement

Defibrinated blood and FD's as desired.

Sterilization

121°C / 15 minutes

Storage

Dry Medium Below 30°C, Prepared Medium 2-8°C.

Directions

Suspend 21.25 grams of MV834 or 19.75 grams of MV834A in 500 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 40 - 50°C and aseptically add 7% v/v sterile defibrinated blood.

For Brucella species: Add rehydrated contents of 1 vial of Brucella Selective Supplement (FD005) to 500 ml sterile molten base.

For Campylobacter species: Add rehydrated contents of 1 vial of Campylobacter Supplement - I (FD006) or Campylobacter Supplement - II (FD007) or Campylobacter Supplement - III (FD008) or Campylobacter Growth Supplement (FD009) to 500 ml sterile molten base.

For Streptococci species: Add rehydrated contents of 1 vial of Strepto Supplement (FD031) to 500 ml sterile molten base. Mix well and pour into sterile petri plates.

Principle and Interpretation

These medias are prepared by using vegetable peptones in place of animal peptones, which make the medium free of BSE/TSE risks. These media can be used to prepare a selective medium for Brucella species or Campylobacter species by adding antibiotic supplement selective for respective bacteria (1, 2) like conventional Blood Agar Base. In comparison to other Blood Agar Base, Blood Agar Base No. 2, HiVeg offers enhanced growth especially for fastidious organisms. Brucella cultures are highly infective and must be handled with care. Incubate preferably in 5-10% carbon dioxide atmosphere. Comparative studies of horse, rabbit and sheep blood were performed by Snavely and Brahier and it was found that sheep blood gave the clearest and most reliable colony and haemolysis characteristics at both 24 and 48 hours (3). These medium can also be used for primary isolation of Haemophilus species, where horse blood is used to enrich the medium. Better results are obtained by spreading half of the horse blood agar plate with 2 drops of 10% saponin (4). Supplementation with blood (5-10%) provides additional growth factors for fastidious microorganisms and is the basis for determining haemolytic reactions. With added HiVeg extract No.2 and yeast extract the medium shows enhanced growth and haemolytic reactions of fastidious organisms like Streptococci and Pneumococci. HiVeg peptone No. 3 is the nitrogen source and sodium chloride maintains osmotic equilibrium.

Quality Control

Appearance of Powder
Yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.

Gelling
Firm, comparable with 1.5% Agar gel of MV834 or 1.2% Agar gel of MV834A.

Colour and Clarity
Basal medium yields yellow coloured clear to slightly opalescent gel, with addition of 7% v/v sterile defibrinated blood, cherry red coloured, opaque gel forms in petri plates.

Reaction
Reaction of 4.25% w/v of MV834 or 3.95% w/v of MV834A aqueous solution is pH 7.4 ± 0.2 at 25°C.

Cultural Response
Cultural characteristics observed after an incubation at 35 - 37°C for 48 hours for MV834 and for 18-48 hours for MV834A.

Organisms(ATCC) Inoculum (CFU) Growth w/blood Recovery Haemolysis
Neisseria meningitidis (13090) 102-103 good to luxuriant >70% none
Streptococcus pneumoniae (6303) 102-103 good to luxuriant >70% alpha
Streptococcus pyogenes (19615) 102-103 good to luxuriant >70% beta
Staphylococcus aureus (25923) 102-103 good to luxuriant >70% beta
More Information
Product Name Blood Agar Base No. 2, HiVeg™
SKU MV834
Product Type HiVeg™
Physical Form Powder
Origin Animal Free (Veg)
Packaging type HDPE
References 1. Norton C. F., 1986, Microbiology, 2nd Edition, Addison-Wesley Publishing Company.2.Hunter D. and Kearns M., 1977, Brit. Vet. J., 133:486.3.Skirrow M. B., 1977, B.M.J., ii: 9.4.Snavely and Brahier, 1960, Am. J. Clin. Pathol., 33:511.5.Waterworth and Pamela M., 1955, Brit. J. Exp. Pathol., 36:186.6.Salfinger Y., and Tortorello M.L. Fifth (Ed.), 2015, Compendium of Methods for the Microbiological Examination of Foods, 5th Ed., American Public Health Association, Washington, D.C.7.U.S. Food and Drug Administration, 1995, Bacteriological Analytical Manual, 8th Ed., AOAC International, Gaithersburg,Md.8.Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)Manual Clinical Microbiology, 11th Edition. Vol. 1.9.Isenberg, H.D. Clinical Microbiology Procedures Handb0ook. 2nd Edition
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