Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for cultivation of anaerobic microorganisms.
Composition
| Ingredients | g / L |
|---|---|
| HiVeg™ hydrolysate | 15.000 |
| L-Cystine | 0.500 |
| Dextrose (Glucose) | 5.500 |
| Yeast extract | 5.000 |
| Sodium chloride | 2.500 |
| Sodium thioglycollate | 0.500 |
| Resazurin | 0.001 |
| Agar | 20.000 |
| Final pH (at 25°C) | 7.1±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 49.0 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Thioglycollate Agar is used for the cultivation of aerobic as well as anaerobic microorganisms in the performance of sterility tests. It is prepared based on the formula specified by US Pharmacopoeia (1) and APHA (2). Thioglycollate Agar is also recommended for the cultivation of Clostridium species (2) and in the culture of Desulfotomaculum nigrificans. Thioglycollate HiVeg™ Agar is prepared by completely replacing animal based peptone with vegetable peptones to avoid BSE/TSE risks associate with animal peptones. HiVeg™ hydrolysate, yeast extract provides nitrogenous and carbonaceous compounds, vitamin B and other essential growth nutrients. Dextrose is the fermentable carbohydrate and energy source. Resazurin is the redox indicator. Thioglycollate neutralizes the bacteriostatic effect of mercurial compounds used as the preservatives in the injection solution. If the solution used in test is a bacteriostatic ingredient then it is necessary to ascertain the bacteriostatic activity of the product.
Type of specimen
Food samples
Specimen Collection and Handling
For food samples, follow appropriate techniques for sample collection and processing as per guidelines (1). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Due to nutritional variations, certain strains may show poor growth.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Gelling: Firm, comparable with 2.0% Agar gel.
Colour and Clarity of prepared medium: Light amber coloured (turning red due to aeration on standing) clear to slightly opalescent gel forms in Petri plates.
Reaction: Reaction of 4.9% w/v aqueous solution at 25°C. pH: 7.1±0.2
pH: 6.90-7.30
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 40-48 hours under anaerobic conditions.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Clostridium botulinum ATCC 25763 | 50-100 | luxuriant | >=50% |
| Clostridium perfringens ATCC 12924 | 50-100 | luxuriant | >=50% |
| Clostridium sporogenes ATCC 11437 | 50-100 | luxuriant | >=50% |
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | Thioglycollate HiVeg™ Agar |
|---|---|
| SKU | MV608 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. The United States Pharmacopoeia, 1985 21st rev. U.S. Pharmacopocial Convention, Rockville, M.D. 2.Speck M. L.(ed.), 1985, Compendium of Methods for the Microbiological examination of Foods, 2nd ed., APHA,Washington, D.C. |
| Customized Product Available | No |
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