Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Perfringens HiVeg Agar Base(O.P.S.P.) with selective supplements is used as a selective medium for isolation and enumeration of Clostridium perfringens in foods.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg hydrolysate | 15.0 |
| Papaic digest of soyabean meal | 5.0 |
| Yeast extract | 5.0 |
| HiVeg extract No. 2 | 7.0 |
| Ferric ammonium citrate | 1.0 |
| Sodium metabisulphite | 1.0 |
| Tris buffer | 1.5 |
| Agar | 15.0 |
| Final pH (at 25°C) | 7.3 ± 0.2 |
Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 25.25 grams in 500 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C. Aseptically add rehydrated contents of 1 vial of Perfringens Supplement I (FD011) and Perfringens Supplement II (FD012) each. Mix well before pouring into sterile petriplates.
Principle and Interpretation
Perfringens HiVeg Agar Base is prepared by using HiVeg hydrolysate and HiVeg extract No.2 which are free from BSE/TSE risks. This medium is the modification of Perfringens Agar Base (O.P.S.P.) which is based on the formula developed by Handford (1) and is used as a selective medium for isolation and enumeration of Clostridium perfringens in foods (2).
HiVeg hydrolysate, yeast extract, papaic digest of soyabean meal and HiVeg extract No.2 supply most of the essential nitrogenous nutrients, vitamin B complex and trace ingredients for the growth of Clostridium perfringens. Sodium metabisulphite and ferric ammonium citrate are used as indicators of sulphite reduction by Clostridium perfringens which produces black colonies. The production of black colonies on this medium is a presumptive identification of Clostridium perfringens, further identification tests must be carried out. Tris buffer helps in maintaining buffering action. The antibiotics Sulphadiazine, Bleandomycin and Polymyxin B make the medium highly selective inhibiting sulphite reducing bacteria other than Clostridium perfringens such as Salmonellae, Bacillus species, Proteus species, Staphylococci etc. Occassional strains of Enterococci will grow on this medium as white colonies, easily distinguished from the large black colonies of Clostridium perfringens.
Product Profile
| Vegetable based (Code MV)© | Animal based (Code M) |
|---|---|
| MV579 | M579 |
| HiVeg hydrolysate | Casein enzymic hydrolysate |
| HiVeg extract No. 2 | Liver extract |
- Recommended for : Isolation and enumeration of Clostridium perfringens in foods
- Reconstitution : 50.5 g/l
- Quantity on preparation (500g) : 9.90 L
- pH (25°C) : 7.3 ± 0.2
- Supplement : Perfringens Supplement I (FD011), Perfringens Supplement II (FD012).
- Sterilization : 121°C / 15 minutes.
- Storage : Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Quality Control
Appearance of powder
Yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling
Firm, comparable with 1.5% Agar gel.
Colour and Clarity
Amber coloured, clear to slightly opalescent gel forms in petri plates.
Reaction
Reaction of 5.05% w/v aqueous solution is pH 7.3 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18 - 48 hours with added Perfringens Supplement I (FD011) and Perfringens Supplement II (FD012).
| Organisms (ATCC) | Inoculum (CFU) | Growth | Recovery | Colour of colony |
|---|---|---|---|---|
| Bacillus subtilis (6633) | 102-103 | inhibited | 0% | - |
| Clostridium bifermentans | 102-103 | inhibited | 0% | - |
| Clostridium butyricum (9690) | 102-103 | inhibited | 0% | - |
| Clostridium perfringens (12924) | 102-103 | luxuriant | >70% | black |
| Enterococcus faecalis (29212) | 102-103 | none-poor | <20% | white, if any |
| Proteus vulgaris (13315) | 102-103 | inhibited | 0% | - |
| Salmonella serotype Typhi (6539) | 102-103 | inhibited | 0% | - |
| Staphylococcus aureus (25923) | 102-103 | inhibited | 0% | - |
| Product Name | Perfringens HiVeg™ Agar Base (O.P.S.P.) |
|---|---|
| SKU | MV579 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Infect. Immun. 61: 3429-3439. 2.Handford P. M., 1974, J. Appl. Bacteriol., 37: 559. 3.Hauschild A. H. W. et al, 1977, ICMSF Methods Studies VIII, Can. J. Microbiol., 23:884. |
| Customized Product Available | No |
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