Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Yeast Malt HiVeg Agar / Broth (YM HiVeg Agar / Broth) is used for the isolation and cultivation of yeasts, moulds and other aciduric microorganisms.
Composition
| Ingredients | MV424 Grams/Litre |
MV425 Grams/Litre |
|---|---|---|
| HiVeg peptone | 5.00 | 5.00 |
| Yeast extract | 3.00 | 3.00 |
| Malt extract | 3.00 | 3.00 |
| Dextrose | 10.00 | 10.00 |
| Agar | 20.00 | — |
| Final pH (at 25°C) | 6.2 ± 0.2 | |
** Formula adjusted, standardized to suit performance parameters
Product Profile
Vegetable based (Code MV)
- MV424/MV425: HiVeg peptone
Animal based (Code M)
- M424/M425: Peptic digest of animal tissue
Recommended for: Isolation and cultivation of yeasts, moulds and other aciduric microorganisms.
Reconstitution
- (MV424): 41.0 g/l
- (MV425): 21.0 g/l
Quantity on preparation
- (500g): (MV424): 12.19 L
- (100g): (MV424): 2.43 L
- (500g): (MV425): 23.8 L
- (100g): (MV425): 4.76 L
pH (25°C): 6.2 ± 0.2
Supplement: None
Sterilization: 121°C / 15 minutes.
Storage: Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Directions
Suspend 41.0 grams of MV424 or 21.0 grams of MV425 in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. For preparing selective media acidify the media upto pH 3.0 to 4.0 or add antibiotics. DO NOT HEAT the media after addition of acid or antibiotics.
Principle and Interpretation
These media are prepared by replacing peptic digest of animal tissue with HiVeg peptone which is free of BSE/TSE risks. Yeast Malt HiVeg Agar/Broth are the modification of Yeast Malt Agar / Broth formulated as per Wickerham (1, 2) for isolation and cultivation of yeasts, moulds and other aciduric microorganisms. Fungistatic materials such as sodium propionate and diphenyl are added to YM HiVeg Agar / Broth to eliminate moulds and thus permits enumeration of yeasts from mixed population.
Yeast Malt HiVeg Broth can be used as an enrichment medium for yeasts by adding a layer of sterile paraffin oil (about 1 cm) on the surface of inoculated broth. After the growth occurs it should be streaked on YM HiVeg Agar to obtain isolated colonies of fermentative species. To isolate fermentative as well as oxidative strains, acidified YM HiVeg Broth is placed on a rotary shaker for 1 or 2 days which favours development of yeast cells while the sporulation of moulds is prevented. Yeasts can be further isolated by streaking on YM Agar.
Quality Control
Appearance of Powder
Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling
Firm, comparable with 2.0% Agar gel of MV424.
Colour and Clarity
Light amber coloured, very slightly opalescent gel forms in petri plates, very slightly opalescent solution in tubes.
Reaction
Reaction of 4.1% w/v of MV424 or 2.1% w/v of MV425 aqueous solution is pH 6.2 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 25-30°C for 40 -72 hours.
| Organisms (ATCC) | Growth at pH 3.4 |
Growth at pH 6.2 |
|---|---|---|
| Aspergillus niger (16404) | good-luxuriant | good-luxuriant |
| Candida albicans (10231) | good-luxuriant | good-luxuriant |
| Saccharomyces cerevisiae (9763) | good-luxuriant | good-luxuriant |
| Lactobacillus leichmannii (4797) | poor | good-luxuriant |
| Escherichia coli (25922) | inhibited | good-luxuriant |
MV424 Yeast Malt HiVeg Agar
- Saccharomyces cerevisiae
- Candida albicans
| Product Name | ISP HiVeg™ Medium No. 2 (Yeast Malt HiVeg™ Agar) (YM HiVeg™ Agar) |
|---|---|
| SKU | MV424 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Wickerham L. J., 1951, U.S. Dept. Agric. Tech. Bull. No.1029. 2.Wickerham L. J., 1939, J. Tropical Med. Hyg., 42:176. 3..Salfinger Y., and Tortorello M.L. Fifth (Ed.), 2015, Compendium of Methods for the Microbiological Examination ofFoods, 5th Ed., American Public Health Association, Washington, D.C. 4.Isenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition. 5.Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)Manual of Clinical Microbiology, 11th Edition. Vol. 1. |
| Customized Product Available | No |
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