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LPM HiVeg™ Agar Base

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SKU:

MV1228

For isolation and cultivation of Listeria monocytogenes from food and dairy products.

Description

LPM (Lithium Phenylethanol Moxalactam) HiVeg Agar Base is recommended for isolation and cultivation of Listeria monocytogenes from food and dairy products.

Composition ** :

Ingredients	Grams/Litre
HiVeg hydrolysate	5.0
HiVeg peptone	5.0
HiVeg extract	3.0
Glycine anhydride	10.0
Lithium chloride	5.0
Sodium chloride	5.0
Phenyl ethyl alcohol	2.5
Agar	15.0

Final pH (at 25°C) 7.3 ± 0.2

** Formula adjusted, standardized to suit performance parameters.

Product Profile :

Vegetable based (Code MV)	Animal based (Code M)
MV1228	M1228
HiVeg hydrolysate	Casein enzymic hydrolysate
HiVeg peptone	Peptic digest of animal tissue
HiVeg extract	Beef extract

Recommended for : Isolation and cultivation of Listeria monocytogenes from food and dairy products,

Reconstitution

Reconstitution: 50.5 g/l

Quantity on preparation (100g): 1.98 L

pH (25°C): 7.3 ± 0.2

Supplement: Moxalactam Supplement (FD151)

Sterilization: 121°C / 12 minutes.

Storage: Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.

Directions :

Suspend 50.5 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 12 minutes. Cool to 50°C and aseptically add rehydrated contents of 1 vial of Moxalactam Supplement (FD151). Mix well before pouring into sterile petri plates.

Caution: Lithium chloride is harmful. Avoid bodily contact and inhalation of vapours. On contact with skin, wash with plenty of water immediately.

Principle and Interpretation :

LPM HiVeg Agar Base is prepared by using HiVeg peptone, HiVeg hydrolysate and HiVeg extract which is free of BSE/TSE risks associated with animal based peptones. LPM HiVeg Agar Base is the modification of LPM Agar Base which is modified McBride Agar developed by Lee and McClain (1) for the isolation of Listeria monocytogenes.

HiVeg hydrolysate, HiVeg peptone and HiVeg extract provides all essential nutrients for metabolism. Glycine anhydride, lithium chloride and phenyl ethyl alcohol suppress gram-positive cocci and gram-negative rods. Moxalactam inhibits both gram-positive and gram-negative bacteria including Staphylococci, Proteus and Pseudomonas species. Listeria monocytogenes show blue-green irridescence when examined with oblique transmitted light (2, 3).

Quality Control :

Appearance of powder

Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.

Gelling

Firm, comparable with 1.5% Agar gel.

Colour and Clarity

Light yellow coloured, clear to slightly opalescent gel forms in petri plates.

Reaction

Reaction of 5.05% w/v aqueous solution is pH 7.3 ± 0.2 at 25°C.

Cultural Response

Cultural characteristics observed after an incubation at 35 - 37°C for 24 - 48 hours.

Organisms (ATCC)

Organism	Inoculum (CFU)	Growth	Recovery
Escherichia coli (25922)	10²-10³	inhibited	0%
Listeria monocytogenes (19111)	10²-10³	good-luxuriant	>50%
Listeria monocytogenes (19112)	10²-10³	good-luxuriant	>50%
Listeria monocytogenes (19117)	10²-10³	good-luxuriant	>50%
Pseudomonas aeruginosa (27853)	10²-10³	inhibited	0%
Staphylococcus aureus (25923)	10²-10³	inhibited	0%

References :

Lee and McClain, 1986, Appl. Environ. Microbiol., 52:1215.
Bearns and Girard, 1959, Am. J. Med. Technol., 25:120.
Borterelssi, Schtech and Albritton, 1985, Manual of Clinical Microbiology, Lennette, Balows, Hausler and Shadomy (Eds.), 4^th ed., ASM, Washington, D.C.

Product Information

More Information
Product Name	LPM HiVeg™ Agar Base
SKU	MV1228
Product Type	HiVeg™
Physical Form	Powder
Origin	Animal Free (Veg)
Packaging type	HDPE
References	1. Lee W. H. and McClain D., 1986, Appl. Environ. Microbiol., 52:1215.2.Downes F. P and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,APHA, Washington, D.C.3.Marshall R. T., (Ed.), 1992, Standard Methods for the Examination of Dairy Products, 16th Ed., APHA, Washington, D.C.4.Bearns R. E. and Girard K. F., 1959, Am. J. Med. Technol., 25:120.5.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,8th Ed., American Society for Microbiology, Washington, D.C.6.Wehr H. M., 1987, J. Assoc. Off. Anal. Chem., 70:769.7.Bremer P. and Osborne C., 1995, J. Food Prot., 58:604.8.Patel J. R., Hwang C. A, Beuchat L. R, Doyle M. P. and Brackett R. E., 1995, J. Food Prot., 58:244
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