Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
MacConkey HiVeg™ Agar w/o CV, w/ 0.003% NR and 1.2% Agar
For selective isolation and differentiation of lactose fermenting and lactose non-fermenting enteric bacteria.
Your enquiry has been submitted
Please login to Post an Enquiry.
Intended use
Recommended for selective isolation and differentiation of lactose non-fermenting from lactose fermenting enteric bacteria.
Composition**
| Ingredients | g/L |
|---|---|
| HiVegⓇpeptone | 17.000 |
| HiVeg® peptone No. 3 | 3.000 |
| Lactose | 10.000 |
| Synthetic detergent | 1.500 |
| Sodium chloride | 5.000 |
| Neutral red | 0.030 |
| Agar | 12.000 |
| Final pH (at 25°C) | 7.1±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 48.53 grams in 1000 ml purified/distilled water. Heat to boiling with gentle swirling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Avoid overheating. Cool to 45-50°C. Mix well and pour into sterile Petri plates. The medium should be dry when inoculated.
Principle And Interpretation
MacConkey Agar Medium is the earliest selective and differential medium for cultivation of enteric microorganisms from a variety of specimens (1,2). MacConkey agars are slightly selective and differential plating media mainly used for the detection and isolation of gram-negative organisms from clinical (3), dairy (4), food (5,6), water (7), and industrial sources (8). It is also recommended for the selection and recovery of the Enterobacteriaceae and related enteric gram-negative bacilli. MacConkey HiVeg® Agar w/o CV w/ 0.003%NR and 1.2% Agar is prepared by completely replacing animal based peptone with vegetable peptones to avoid BSE/TSE risks associate with animal peptones. This medium has HiVeg® peptone and HiVeg® peptone no. 3 which provides necessary nitrogen sources for growth of organisms. The selective action is due to synthetic detergent in the medium. Lactose fermenting strains grow as pink to red colonies and may be surrounded by a zone of acid precipitated bile. The pink to red colour is due to production of acid from lactose, absorption of neutral red and a subsequent colour change of the dye due to pH drop of medium. Lactose non-fermenting strains, such as Shigella and Salmonella are colorless and transparent and typically do not alter appearance of the medium. Sodium chloride in the medium helps to maintain osmotic balance of the cells.
Type of specimen
Food and dairy samples; Water samples.
Specimen Collection and Handling
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (4-6). For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards.(7) After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Although this medium is selective for gram negative organisms, biochemical identification and serological testing using pure cultures is recommended for complete identification.
- It is advised to incubate for recommended period and temperature to avoid misinterpretation of results.
- It is advised to read the results immediately after incubation, as overgrowth of Proteus species may mask other colonies.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Light yellow to pink homogeneous free flowing powder
Gelling
Firm, comparable with 1.2% Agar gel.
Colour and Clarity of prepared medium
Orange red coloured clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 4.85% w/v aqueous solution at 25°C. pH: 7.1±0.2
pH
6.90-7.30
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of Colony |
|---|---|---|---|---|
| Escherichia coli ATCC 25922 (00034*) | 50-100 | luxuriant | >=50% | pink to red with bile precipitate |
| # Klebsiella aerogenes ATCC 13048 (00175*) | 50-100 | luxuriant | >=50% | pink to red |
| Enterococcus faecalis ATCC 29212 (00087*) | 50-100 | fair | 30-40% | pale pink to red |
| $ Proteus hauseri ATCC 13315 | 50-100 | luxuriant | >=50% | colourless |
| Salmonella Paratyphi A ATCC 9150 | 50-100 | luxuriant | >=50% | colourless |
| Shigella flexneri ATCC 12022 (00126*) | 50-100 | luxuriant | >=50% | colourless |
| Salmonella Paratyphi B ATCC 8759 | 50-100 | luxuriant | >=50% | colourless |
| Salmonella Enteritidis ATCC 13076 (00030*) | 50-100 | luxuriant | >=50% | colourless |
| Salmonella Typhi ATCC 6539 | 50-100 | luxuriant | >=50% | colourless |
| Staphylococcus aureus susp.aureus ATCC 25923 (00034*) | >=104 | inhibited | 0% |
Key: *Corresponding WDCM numbers
#- Formerly known as Enterobacter aerogenes.
$- Formerly known as Proteus vulgaris
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (9,10).
| Product Name | MacConkey HiVeg™ Agar w/o CV, w/ 0.003% NR and 1.2% Agar |
|---|---|
| SKU | MV008B |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg), Lactose |
| Packaging type | HDPE |
| References | 1. MacConkey, 1900, The Lancet, ii:20. 2.MacConkey, 1905, J. Hyg., 5:333. 3.Salfinger Y., and Tortorello M.L. Fifth (Ed.), 2015, Compendium of Methods for the Microbiological Examination ofFoods, 5th Ed., American Public Health Association, Washington, D.C. 4.Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water and Wastewater,23rd ed., APHA, Washington, D.C. 5.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,APHA Inc., Washington, D.C. 6.Isenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition. 7.Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)Manual of Clinical Microbiology, 11th Edition. Vol. 1. |
| Customized Product Available | No |
Write Your Own Review
MacConkey Agar w/o CV, w/ 1.2% Agar
As low as
₹4,732.00
Upsell Products
HiEncap™ MacConkey Agar w/0.15% Bile Salts, CV and NaCl
As low as
₹0.00
HiEncap™ MacConkey Agar w/o CV, NaCl w/0.5% Bile Salts
As low as
₹0.00
MacConkey Agar
As low as
₹1,174.00
MacConkey Agar Plate
As low as
₹6,360.00
HiCombi™ Nutrient - MacConkey Agar Plate
As low as
₹2,366.00
HiCombi™ MacConkey - MacConkey Agar Plate
As low as
₹2,696.00
MacConkey Agar w/ 0.15% Bile Salts, CV and NaCl Plate
As low as
₹1,670.00
MacConkey Agar w/o CV, NaCl w/ 0.5% Sodium Taurocholate Plate
As low as
₹1,670.00
MacConkey Agar Plate
As low as
₹1,746.00
MacConkey Agar Plate (Triple Pack)
As low as
₹1,469.00