Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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MacConkey Agar Plate
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$221.10
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SKU:
SPH081
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Intended Use
Recommended for selective isolation and differentiation of E.coli and other enteric bacteria from pharmaceutical products in accordance with the microbial limit testing by harmonized methodology of USP/EP/BP/JP.
Composition
| Ingredients | g/L |
|---|---|
| Gelatin peptone # | 17.000 |
| HMC peptone ## | 3.000 |
| Lactose monohydrate | 10.000 |
| Sodium chloride | 5.000 |
| Bile salts | 1.500 |
| Neutral red | 0.030 |
| Crystal violet | 0.001 |
| Agar | 13.500 |
| pH after sterilization (at 25°C) | 7.1±0.2 |
**Formula adjusted, standardized to suit performance parameters
# Pancreatic digest of gelatin
## Peptones (meat and casein)
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
MacConkey Agar is the earliest selective and differential medium for cultivation of coliform organisms (1,2). Subsequently MacConkey Agar and Broth have been recommended for use in microbiological examination of foodstuffs (3) and for direct plating / inoculation of water samples for coliform counts (4). This medium is also accepted by the Standard Methods for the Examination of Milk and Dairy Products (5). It is recommended in pharmaceutical preparations and is in accordance with the harmonized method of USP/EP/BP/JP (6-9).
Gelatin peptone and HMC peptone provide the essential nutrients, vitamins and nitrogenous factors required for growth of microorganisms. Lactose monohydrate is the fermentable source of carbohydrate. The selective action of this medium is attributed to crystal violet and bile salts, which are inhibitory to most species of gram-positive bacteria. Sodium chloride maintains the osmotic balance in the medium.
After enrichment of Escherichia coli in MacConkey Broth (MH083), it is then subcultured on MacConkey Agar. Gram-negative bacteria usually grow well on the medium and are differentiated by their ability to ferment lactose. Lactose fermenting strains grow as red or pink and may be surrounded by a zone of acid precipitated bile. The red colour is due to production of acid from lactose, absorption of neutral red and a subsequent colour change of the dye when the pH of medium falls below 6.8. Lactose non-fermenting strains, such as Shigella and Salmonella are colourless and transparent and typically do not alter appearance of the medium. Yersinia enterocolitica may appear as small, non-lactose fermenting colonies after incubation at room temperature.
Type of specimen
Pharmaceutical samples, Food and dairy samples; Water samples.
Specimen Collection and Handling
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (3,5).
For water samples, follow appropriate techniques for sample collection and processing as per guidelines (4).
For pharmaceutical samples, follow appropriate techniques for sample collection, processing as per guidelines (6-9).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the pack. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- It is recommended to store the plates at 24-30°C to avoid minimum condensation.
- Though the medium is recommended for selective isolation, further biochemical and serological testing must be carried out for complete identification.
- The surface of the medium should be dry when inoculated.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Sterile MacConkey Agar in 55mm scored disposable plates with convex surface and absence of black particles/ cracks/ bubbles.
Colour of medium: Red with purplish tinge coloured medium
Quantity of medium: 20 ml of medium in 55 mm scored disposable plates.
pH: 6.90-7.30
Sterility Check: Passes release criteria
Cultural Response
Growth Promotion is carried out in accordance with the harmonized method of ICH (USP/EP/BP/JP). Cultural response was observed after an incubation at 30-35°C for 18-72 hours. Recovery rate is considered as 100% for bacteria growth on Soybean Casein Digest Agar.
Growth promoting properties: Growth of microorganism comparable to that previously obtained with previously tested and approved lot of medium occurs at the specified temperature for not more than the shortest period of time specified inoculating 100 cfu (at 30-35°C for<=18 hours).
Indicative properties: Colonies are comparable in appearance and indication reaction to those previously obtained with previously tested and approved lot of medium occurs for the specified temperature for a period of time within the range specified inoculating <=100 cfu (at 30-35°C for 18-72 hours).
| Organism | Inoculum (CFU) | Growth | Observed Lot | Recovery value (CFU) | Colour of colony | Incubation period |
|---|---|---|---|---|---|---|
| Growth Promoting + Indicative | ||||||
| Escherichia coli ATCC 8739 (00012*) | 50-100 | luxuriant | 25-100 | >=50% | pink-red with bile precipitate | 18-72 hrs |
Key :- (*) Corresponding WDCM numbers,
Storage and Shelf Life
On receipt store between 20-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
| Product Name | MacConkey Agar Plate |
|---|---|
| SKU | SPH081 |
| Customized Product Available | No |
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