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HiCrome™ EC Broth w/RUG

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M2073

For detection of Escherichia coli in water and food samples by a chromogenic and fluorogenic method.

Description

Escherichia coli can be reliably detected with this media that contain a chromogenic or fluorogenic substrate for beta-glucuronidase, an enzyme that occurs almost exclusively in E. coli . Resorufin-beta-D-glucuronic acid methyl ester (RUG) is a highly sensitive chromogenic and fluorogenic indicator for Escherichia coli RUG is more specific and does not require fluorescent detection. The released dye Resorufin itself gives intense pink color which can be visually detected. Additional confirmation can be done by observation of fluorescence under uv light .

Product Information

More Information
Product Name	HiCrome™ EC Broth w/RUG
SKU	M2073
Product Type	HiCrome™
Physical Form	Powder
Origin	Animal
Packaging type	HDPE
References	1.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,8th Ed., American Society for Microbiology, Washington, D.C. ,,2.Koneman E. W., Allen S. D., Janda W. M., Schreckenberger P. C., Winn W. C. Jr., 1992, Colour Atlas and Textbook ofDiagnostic Microbiology, 4 th Ed., J. B. Lippinccott Company3.Meyer and Schonfeld, 1926, Zentralbl. Bakeriol, Parasitenk. Infectionskr. Hyg. Abt. Orig. 99:402.4.Rochaix, 1924, Comt. Rend. Soc. Biol., 90:771.5.American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed., WashingtonD.C.6.Salfinger Y., and Tortorello M.L. Fifth (Ed.), 2015, Compendium of Methods for the Microbiological Examination ofFoods, 5th Ed., American Public Health Association, Washington, D.C.7.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,APHA Inc., Washington, D.C.8.Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water and Wastewater,23rd ed., APHA, Washington, D.C.9.Isenberg, H.D. Clinical Microbiology Procedures Handb0ook. 2nd Edition.10.Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)Manual of Clinical Microbiology, 11th Edition. Vol. 1.
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