Alicyclobacillus Agar, Granulated, BAT Agar, Granulated

Composition**

Ingredients	Gms / Litre
Yeast extract	2.000
Dextrose	5.000
Calcium chloride	0.25066
Magnesium sulphate	0.500
Ammonium sulphate	0.200
Potassium dihydrogen phosphate	3.000
Zinc sulphate	0.00018
Copper sulphate	0.00016
Manganese sulphate	0.00015
Cobalt chloride	0.00018
Boric acid	0.00010
Sodium molybdate	0.00030
Agar	18.000
Final pH (at 25°C)	4.0±0.2

**Formula adjusted, standardized to suit performance parameters

Directions

Suspend 28.95 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C.Mix well and pour into sterile Petri plates.

Note: Adjust the pH of the medium to 4.0 ±0.2 (after sterilization) using IN H₂SO₄ or 1N NaOH.

Principle And Interpretation

Alicyclobacillus species are gram positive aerobic thermophillic, and spore forming acidophilic bacteria. Alicyclobacillus are sometimes called Acidophilic Thermophillic Bacteria (ATB). These spore forming organisms are able to survive the relatively mild pasteurization temperatures used for fruit juices and drinks and some are able to grow out and cause spoilage of the beverage. Even very low numbers of Alicyclobacillus are able to cause spoilage and produce objectionable flavours and odours specially affecting the quality of fruit juice (1,2) and in the beverages, damaging the brand. These bacteria are able to grow at pH values as low as 2.5 and also at elevated temperatures as high as 60°C.

Alicyclobacillus Agar has a pH of 4.0 ± 0.2 which inhibits most of the microbial flora. Rest of the microbial flora is inhibited at 60°C, which is the optimum growth temperature for Alicyclobacillus species. Alicyclobacillus Agar is a slight modification of the medium recommended for the cultivation of Alicyclobacillus acidoterrestris (3).

Quality Control

Appearance
Cream to yellow coloured granular medium

Gelling
Firm, comparable with 1.8% Agar gel

Colour and Clarity of prepared medium
Light amber coloured clear to slightly opalescent gel forms in Petri plates

Reaction
Reaction of 2.89% w/v aqueous solution at 25°C. pH: 4.0±0.2

pH
3.80-4.20

Cultural Response

Cultural characteristics observed after an incubation at 60°C for 48-72 hours.

Organism	Growth
Alicyclobacillus acidocaldarius 27009	good to luxuriant
Escherichia coli ATCC 25922	inhibited
Staphylococcus aureus ATCC 25923	inhibited
Saccharomyces cerevisiae ATCC 19615	inhibited
Candida albicans ATCC 10231	inhibited

Storage and Shelf Life

Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.

Product Information

More Information
Product Name	Alicyclobacillus Agar, Granulated (BAT Agar, Granulated)
SKU	GM1650
Product Type	Granulated
Physical Form	Granular
Origin	Animal
Packaging type	HDPE
References	1.Ceny G., Hennlish W. and K Rocallia-Furchtsaftwerb ducrh Baciilen. Isobioerung andCharakteriseeuing des Verdebserregens-Z hebers Utres Forsch 179: 224-227, 1984.2.Baungart and Merve S., The Impact of Alicyclobacillus acidoterstris on the Quality of Juicesand Soft Drinks Fruit processing 7: 251-254 (2000).3.Atlas R. M., 2010, Handbook of Microbiological Media, 4th Ed, CRC Press.
Customized Product Available	No