Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Bromocresol Purple Broth with Lactose, granulated is used for identification of Escherichia coli and coliform bacteria from water samples.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Peptic digest of animal tissue | 10.000 |
| Meat extract | 3.000 |
| Sodium chloride | 5.000 |
| Lactose | 10.000 |
| Bromo cresol purple | 0.020 |
| Final pH (at 25°C) | 7.2±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 28.02 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense in tubes containing Durhams tubes and sterilize by autoclaving at 115°C for 20 minutes.
Principle And Interpretation
The coliform group of bacteria is the principal indicator of suitability of water for domestic, industrial or other uses. The coliform group density has been established as a criterion of the degree of pollution and thus of sanitary quality. Faecal Streptococci and Enterococci are also indicators of faecal pollution (1). Where it is claimed that drinking water has been processed for safety, the finding of such organism demonstrates a failure of the process. It is a valuable bacterial indicator for determining the extent of faecal contamination of recreational surface waters or drinking water (2).
Bromo Cresol Purple Broth with Lactose is used for the identification of Escherichia coli and coliforms from water. It is used for enrichment and determining the titre of coliforms in the bacteriological analysis of drinking water (3, 4).
The medium contains peptic digest of animal tissue and meat extract, which supplies the essential nutrients for E. coli and other coliforms. Sodium chloride maintains the osmotic equilibrium of the medium. Lactose upon fermentation by coliforms produce acid and is indicated by the pH indicator bromo cresol purple. It turns yellow at acidic pH.
Quality Control
Appearance: Cream to yellow coloured granular medium
Colour and Clarity of prepared medium: Purple coloured clear solution without any precipitate
Reaction: Reaction of 2.8% w/v aqueous solution at 25°C. pH: 7.2±0.2
pH
7.00-7.40
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Acid | Gas |
|---|---|---|---|---|
| Escherichia coli ATCC 25922 | 50-100 | good-luxuriant | positive reaction, yellow colour | positive reaction |
| Enterococcus faecalis ATCC 29212 | 50-100 | fair-good | variable reaction | negative reaction |
| Klebsiella pneumoniae ATCC 13883 | 50-100 | good-luxuriant | positive reaction, yellow colour | positive reaction |
| Salmonella Typhimurium ATCC 14028 | 50-100 | good-luxuriant | negative reaction, no colour change | negative reaction |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Bromo Cresol Purple Broth w/ Lactose, Granulated |
|---|---|
| SKU | GM1265 |
| Product Type | Granulated |
| Physical Form | Granular |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Eaton A. D., Clesceri L. S. and Greenberg A. E., (Eds), 1998, Standard Methods for the Examination of Water and Wastewater, 20th Ed, APHA, Washington, D.C. |
| Customized Product Available | No |
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