Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
Luria Bertani Agar, Modified, Granulated
Luria Bertani Agar, Modified, granulated is used for the cultivation and maintenance of recombinant strains of Escherichia coli and may be used for routine cultivation of not particularly fastidious microorganisms
Your enquiry has been submitted
Please login to Post an Enquiry.
Composition
| Ingredients | Gms/Litre |
|---|---|
| Casein enzymic hydrolysate | 10.000 |
| Yeast extract | 5.000 |
| Sodium chloride | 5.000 |
| Agar | 10.000 |
| Final pH (at 25°C) | 7.2±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 30 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates or as desired.
Principle And Interpretation
Luria Bertani Agar, Modified is prepared as described by Lennox (1) for cultivation and maintenance of recombinant strains of Escherichia coli. The medium is nutritionally rich for the growth of pure cultures of recombinant strains. Strains generally derived from Escherichia coli K12 are deficient in Vitamin B synthesis which are further modified by specific mutation to create auxotrophic strains and are unable to grow on nutritionally deficient media.
Casein enzymic hydrolysate provides peptides and peptones while Vitamin B complex is provided by yeast extract. Sodium chloride provides sodium ions for the membrane transport and maintains osmotic equilibrium of the medium.
Quality Control
Appearance: Cream to yellow coloured granular medium
Gelling: Firm, comparable with 1.0% Agar gel.
Colour and Clarity of prepared medium: Yellow to amber Clear to slightly opalescent gel forms in Petri plates
Reaction: Reaction of 3.0% w/v aqueous solution at 25°C. pH: 7.2±0.2
pH: 7.00-7.40
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
Cultural Response
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Escherichia coli ATCC 23724 | 50-100 | luxuriant | >=70% |
| Escherichia coli ATCC 25922 | 50-100 | luxuriant | >=70% |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.
Reference
- Lennox E.S., 1955, Transduction of Linked Genetic Characters of the host by bacteriophage P1., Virology, 1:190.
- Atlas R.M., 1993, Handbook of Microbiological Media, Ed. by Parks L., CRC Press, Inc.
| Product Name | Luria Bertani Agar, Modified, Granulated |
|---|---|
| SKU | GM1151F |
| Product Type | Granulated |
| Physical Form | Granular |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Lennox E.S., 1955, Transduction of Linked Genetic Characters of the host by bacteriophage P1., Virology, 1:190. |
| Customized Product Available | No |
Write Your Own Review
Tartoff - Hobbs HiVeg™ Broth (Terrific HiVeg™ Broth)
As low as
₹8,189.00
Luria Bertani Agar, Miller, Granulated (Miller Luria Bertani Agar, Granulated)
As low as
₹6,661.00
Tartoff - Hobbs Broth, Granulated (Terrific Broth, Granulated)
As low as
₹8,950.00
Luria Bertani Agar, Miller (Miller Luria Bertani Agar)
As low as
₹0.00
Luria Bertani Agar, Modified
As low as
₹6,360.00
Luria Bertani Broth, Miller (Miller Luria Bertani Broth)
As low as
₹0.00
Tartoff - Hobbs Broth (Terrific Broth)
As low as
₹7,794.00
Tartoff - Hobbs Broth (Terrific Broth) (γ-Irradiated)
As low as
₹9,062.00
Luria Bertani Broth, Miller pH 7.0
As low as
₹5,275.00
Luria Bertani Agar, Miller pH 7.0
As low as
₹5,257.00
Luria Bertani HiCynth™ Agar, Miller (Miller Luria Bertani HiCynth™ Agar)
As low as
₹6,101.00
Luria Bertani HiCynth™ Broth, Miller (Miller Luria Bertani HiCynth™ Broth)
As low as
₹5,817.00
Luria Bertani HiVeg™ Agar, Miller (Miller Luria Bertani HiVeg™ Agar)
As low as
₹6,101.00
Upsell Products
Luria Bertani Agar Plate w/100µg/ml Ampicillin
As low as
₹2,100.00
Luria Bertani Agar Plate w/ IPTG
As low as
₹2,213.00
Luria Bertani Agar Plate w/100µg/ml Kanamycin
As low as
₹2,083.00
Luria Bertani Agar Plate w/100µg/ml Kanamycin & IPTG
As low as
₹2,319.00
