Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
Your enquiry has been submitted
Something problem with the server. Try again.
Tartoff - Hobbs HiVeg™ Broth (Terrific HiVeg™ Broth)
As low as
$113.30
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MV1250
For the cultivation of recombinant strains of Escherichia coli.
Your enquiry has been submitted
Please login to Post an Enquiry.
Intended Use
Tartoff - Hobbs HiVeg Broth (Terrific HiVeg Broth) with added glycerol is recommended for cultivation of recombinant strains of Escherichia coli.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg hydrolysate | 12.0 |
| Yeast extract | 24.0 |
| Monopotassium phosphate | 9.4 |
| Dipotassium phosphate | 2.2 |
Final pH (at 25°C) 7.2 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Product Information
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV1250 | M1250 |
| HiVeg hydrolysate | Casein enzymic hydrolysate |
Directions
Suspend 47.6 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Add 4 ml of glycerol to the medium. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Technical Data
| Reconstitution | 47.6 g/l |
| Quantity on preparation (500g) | 10.50 L |
| Supplement | Glycerol |
| Sterilization | 121°C / 15 minutes. |
Principle and Interpretation
This medium is prepared by using HiVeg hydrolysate which is free from BSE/TSE risks associated with animal based peptone. Terrific HiVeg Broth is the modification of Terrific Broth which is a very nutritious medium designed by Tartoff and Hobbs (1) to improve the yield of plasmid in Escherichia coli. Sambrook et al (2) further recommended the protocol for the inoculation and incubation of organisms. HiVeg hydrolysate and yeast extract supply the necessary nutrients and cofactors for the excellent growth of recombinant strains of Escherichia coli. Recombinant strains have an extended growth phase in this medium. High concentration of yeast extract and HiVeg hydrolysate in the medium facilitates higher cell yields. Phosphates provide good buffering action to the medium and prevent cell death due to a drop in pH. Glycerol serves as the carbon and energy source to the organisms. Unlike glucose, glycerol is not fermented to acetic acid.
Quality Control
Appearance of powder
Yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Colour and Clarity
Light amber coloured, clear solution without any precipitate.
Reaction
Reaction of 4.76% w/v aqueous solution is pH 7.2 ± 0.2 at 25°C
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth* |
|---|---|---|
| Escherichia coli (23724) | 102-103 | good |
| Escherichia coli (39403) | 102-103 | good |
| Escherichia coli (47014) | 102-103 | good |
| Escherichia coli (53868) | 102-103 | good |
Key: * = growth is evident by turbidity
Storage
Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
References
- Tartoff K.D. and Hobbs C.A., 1987, Improved Media for Growing Plasmid and Cosmid Clones, Bethesda Res. Lab. Focus, 9:12.
- Sambrook J., Fritsch, E.E., and Maniatis T., 1989, Molecular cloning :- A laboratory manual, 2nd ed., Cold Spring Harbor Lab., Cold Spring Harbor, N.Y.
| Product Name | Tartoff - Hobbs HiVeg™ Broth (Terrific HiVeg™ Broth) |
|---|---|
| SKU | MV1250 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Tartoff K. D. and Hobbs C. A., 1987, Improved Media for Growing Plasmid and Cosmid Clones, Bethesda Res. Lab. Focus,9:12. |
| Customized Product Available | No |
Write Your Own Review
Tartoff - Hobbs Broth, Granulated (Terrific Broth, Granulated)
As low as
$170.50
Tartoff - Hobbs Broth (Terrific Broth)
As low as
$113.30