Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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HiEncap™ Luria Bertani Agar, Miller (HiEncap™ Miller Luria Bertani Agar)
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SKU:
EC1151CCL
For the cultivation and maintenance of recombinant strains of Escherichia coli, for genetic and molecular biology studies and cultivation of other nonfastidious microorganisms.
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Intended Use
Recommended for the cultivation and maintenance of recombinant strains of Escherichia coli for genetic and molecular biology studies and cultivation of other non fastidious microorganisms.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Tryptone | 10.000 |
| Yeast extract | 5.000 |
| Sodium chloride | 10.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.5±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Each capsule contains 10 gms of medium. Suspend 1 capsule in 250 ml (4 capsules in 1000 ml) distilled or purified water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Luria Bertani Agar is prepared as described by Lennox (4) for cultivation and maintenance of recombinant strains of Escherichia coli. Luria Bertani Agar, Miller (1) is slightly different with double amount of sodium chloride. The media is nutritionally rich for the growth of pure cultures of recombinant strains. Strains derived from Escherichia coli K12 are deficient in Vitamin B synthesis are further modified by specific mutation to create auxotrophic strains and are therefore unable to grow on nutritionally deficient media. Tryptone provides peptides and peptones while Vitamin B complex is provided by yeast extract. Sodium chloride provides sodium ions for membrane transport and also maintains the osmotic equilibrium of the medium.
Type of specimen
Recombinant strains of E.coli
Specimen Collection and Handling
For recombinant strain samples follow appropriate techniques for handling specimens as per established guidelines (1,4). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Some strains may show poor growth due to nutritional variations.
- Further biochemical and serological tests must be carried out for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance Gelatin capsule containing cream to yellow coloured granular media
Gelling Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium Yellow to amber coloured, clear to slightly opalescent gel forms in Petri plates
Quantity Each capsule contains 10 grams of medium sufficient for 250 ml media
Reaction Reaction of 4.0% w/v aqueous solution at 25°C. pH: 7.5±0.2
pH 7.30-7.70
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Growth | Inoculum (CFU) |
Recovery |
|---|---|---|---|
| Escherichia coli ATCC 23724 | luxuriant | 50-100 | >=70% |
| Escherichia coli ATCC 25922 (00013*) | luxuriant | 50-100 | >=70% |
| Escherichia coli DH5 alpha MTCC 1652 | luxuriant | 50-100 | >=70% |
Key: (*) Corresponding WDCM numbers
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3).
| Product Name | HiEncap™ Luria Bertani Agar, Miller (HiEncap™ Miller Luria Bertani Agar) |
|---|---|
| SKU | EC1151CCL |
| Product Type | HiEncap™ |
| Physical Form | Granulated Media in Gelatin Capsule |
| Origin | Animal |
| Packaging type | HDPE Jar |
| References | 1.Lennox E.S., 1955, Transduction of Linked Genetic Characters of the host by bacteriophage P1., Virology, 1:190. 2. Atlas R.M., 1993, Handbook of Microbiological Media, Ed. by Parks L., CRC Press, Inc. |
| Customized Product Available | No |
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