Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Luria Bertani Agar, Miller, Granulated (Miller Luria Bertani Agar, Granulated)
Rich medium with high salt content , recommended for recombinat strains of E. Coli
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Composition
| Ingredients | Gms/Litre |
|---|---|
| Casein enzymic hydrolysate | 10.000 |
| Yeast extract | 5.000 |
| Sodium chloride | 10.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.5±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 40 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and and pour into sterile Petri plates or as desired.
Principle And Interpretation
Luria Bertani Agar is prepared as described by Lennox (1) for cultivation and maintenance of recombinant strains of Escherichia coli. Luria Bertani Agar, Miller (2) is slightly different with double amount of sodium chloride. The media is nutritionally rich for the growth of pure cultures of recombinant strains. Strains derived from Escherichia coli K12 are deficient in Vitamin B synthesis are further modified by specific mutation to create auxotrophic strains and are therefore unable to grow on nutritionally deficient media.
Casein enzymic hydrolysate provides peptides and peptones while Vitamin B complex is provided by yeast extract. Sodium chloride provides sodium ions for membrane transport and also maintains the osmotic equilibrium of the medium.
Quality Control
Appearance
Cream to yellow coloured granular medium
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Yellow to amber coloured, clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 4.0% w/v aqueous solution at 25°C. pH : 7.5±0.2
pH
7.30-7.70
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Escherichia coli ATCC 23724 | 50-100 | luxuriant | >=70% |
| Escherichia coli ATCC 25922 | 50-100 | luxuriant | >=70% |
| Escherichia coli DH5 alpha MTCC 1652 | 50-100 | luxuriant | >=70% |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.
Reference
- Lennox E.S., 1955, Transduction of Linked Genetic Characters of the host by bacteriophage P1., Virology, 1:190.
- Atlas R.M., 1993, Handbook of Microbiological Media, Ed. by Parks L., CRC Press, Inc.
| Product Name | Luria Bertani Agar, Miller, Granulated (Miller Luria Bertani Agar, Granulated) |
|---|---|
| SKU | GM1151 |
| Product Type | Granulated |
| Physical Form | Granular |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Lennox E.S., 1955, Transduction of Linked Genetic Characters of the host by bacteriophage P1., Virology, 1:190. |
| Customized Product Available | No |
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