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HiEncap™ Sabouraud Dextrose Broth (HiEncap™ Sabouraud Liquid Medium)

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EC033D

For cultivation of yeasts, moulds and aciduric microorganisms.

Description

Intended Use

Recommended for cultivation of yeasts, moulds and aciduric microorganisms.

Composition

Ingredients	Gms / Litre
Dextrose (Glucose)	20.000
Special peptone	10.000
Final pH (at 25°C)	5.6±0.2

Formula adjusted, standardized to suit performance parameters

Directions

Each capsule contains 15 grams of media. Suspend 1 capsule in 500 ml (2 capsules in 1000 ml) distilled or purified water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and dispense as desired.

Principle And Interpretation

Sabouraud Dextrose Agar is Carliers modifications (2) of the formulation described by Sabouraud (6) for the cultivation of fungi, particularly those associated with skin infections. The medium is also recommended by APHA (8). Sabouraud Dextrose Broth is also a modification by Sabouraud (7) and serves the same purpose as Sabouraud Dextrose Agar.

Sabouraud dextrose media are peptone media supplemented with dextrose to support the growth of fungi. Peptone special provides nitrogen, vitamins, minerals, amino acids and growth factors. Dextrose provides an energy source for the growth of microorganisms. The low pH favours fungal growth and inhibits contaminating bacteria from clinical specimens (5). The acid reaction of the final medium is inhibitory to a large number of bacteria making it particularly useful for cultivating fungi and aciduric microorganisms. For isolation of fungi from contaminated specimens, a selective medium should be inoculated simultaneously. Incubate cultures for 4 to 6 weeks before reporting as negative.

Type of specimen

Clinical: skin scrapings; Pharmaceutical samples.

Specimen Collection and Handling

For clinical samples follow appropriate techniques for handling specimens as per established guidelines (3,4). For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (1,8). After use, contaminated materials must be sterilized by autoclaving before discarding.

Warning and Precautions

In Vitro diagnostic use. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.

Limitations

Since it is a general purpose medium, bacterial cultures will also grow.
Incubate cultures for 4 to 6 weeks before reporting as negative.
Further isolation and biochemical tests should be carried out for confirmation.

Performance and Evaluation

Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.

Quality Control

Appearance: Gelatin capsule containing cream to yellow coloured granular media

Colour and Clarity of prepared medium: Light amber coloured clear solution in tubes

Quantity: Each capsule contains 15 grams of medium sufficient for 500 ml media

Reaction: pH of 3.0% w/v aqueous solution at 25°C. pH : 5.6±0.2

pH: 5.40-5.80

Cultural Response

Cultural characteristics was observed after an incubation at 20-25°C for 3-5 days.

Organism	Inoculum (CFU)	Growth	Incubation temperature	Incubation period
Candida albicans ATCC 10231 (00054*)	50-100	luxuriant	20-25 °C	<=5 d
Aspergillus brasiliensis ATCC 16404 (00053*)	50-100	luxuriant	20-25 °C	<=5 d
Saccharomyces cerevisiae ATCC 9763 (00058*)	50-100	luxuriant	20-25 °C	3-5 d
Saccharomyces cerevisiae ATCC 2601	50-100	good-luxuriant	20-25 °C	3-5 d
Candida albicans ATCC 2091 (00055*)	50-100	luxuriant	20-25 °C	3-5 d
Escherichia coli ATCC 8739 (00012*)	50-100	Luxuriant (inhibited on media with low pH)	20-25 °C	<=5 d
Escherichia coli ATCC 25922 (00013*)	50-100	good-luxuriant	20 -25 °C	3-5 d
Escherichia coli NCTC 9002	50-100	Luxuriant (inhibited on media with low pH)	20-25 °C	3-5 d
Lactobacillus casei ATCC 334	50-100	luxuriant	20-25 °C	3-5 d

Key: *Corresponding WDCM numbers.

Storage and Shelf Life

Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.

Disposal

User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4)

Product Information

More Information
Product Name	HiEncap™ Sabouraud Dextrose Broth (HiEncap™ Sabouraud Liquid Medium)
SKU	EC033D
Application/ Industry	Clinical
Product Type	HiEncap™
Physical Form	Granulated Media in Gelatin Capsule
Origin	Animal
Packaging type	HDPE Jar
References	1. Carlier G. I. M., 1984, Brit. J. Derm. Syph., 60:61 2. Sabouraud R., 1892, Ann. Dermatol. Syphil. 3 : 1061. 3. Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., APHA, Washington, D.C. 4. Sabouraud R., Les Teignes, Paris: Masson et Cie, 1910, p 5535.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology, 8th Ed., American Society for Microbiology, Washington, D.C.
Customized Product Available	No