HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Intended Use
Preston HiVeg Agar Base with added supplement is recommended for selective isolation of thermotolerant Campylobacter species.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg peptone | 10.0 |
| HiVeg extract | 10.0 |
| Sodium chloride | 5.0 |
| Agar | 12.0 |
Final pH (at 25°C) 7.5 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 18.5 grams in 470 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add 25 ml sterile, lysed horse blood and reconstituted contents of Campylobacter Selective Supplement IV (FD042). Mix well and pour plates.
Principle and Interpretation
Preston HiVeg Agar Base is prepared by completely replacing animal based peptones with vegetable peptones that make the medium free of BSE/TSE risks. Preston HiVeg Agar Base is the modification of medium described by Bolton and Robertson (1) for isolation of Campylobacter species and is also recommended by APHA (2). Isolation of Campylobacter species on selective agar medium is made both with or without selective broth enrichment. Direct plating without enrichment is adequate for fresh faecal samples, or intestinal specimens as high numbers of the organisms may be anticipated. For food samples enrichment is required.
Campylobacter species grow well in microaerobic conditions i.e. in 5% O2 (Oxygen) at 42°C in about 48 hours. Typically on moist media, C. jejuni growth swarms, which is a useful diagnostic growth characteristic, however, this type of confluent growth makes it difficult to obtain isolated colonies. Addition of about 4 drops of glycerol to a filter paper kept within the jar/container will hamper confluent and swarming growth of Campylobacter (3). The antibiotic supplement renders it selective for Campylobacter species.
Quality Control
Appearance of powder: Yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV939 | M939 |
| HiVeg peptone | Peptic digest of animal tissue |
| HiVeg extract | Beef extract |
Recommended for
Selective isolation of thermotolerant Campylobacter species.
Reconstitution
| Quantity on preparation (500g) | 13.51 L |
| pH (25°C) | 7.5 ± 0.2 |
| Supplement | Lysed horse blood, Campylobacter Selective Supplement IV (FD042) |
Sterilization
121°C / 15 minutes.
Storage
Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Gelling
Firm, comparable with 1.2% Agar gel.
Colour and Clarity
Basal medium forms light yellow coloured clear to slightly opalescent gel. With addition of sterile lysed horse blood, chocolate brown coloured opaque gel forms in the petri plates.
Reaction
Reaction of 3.7% w/v aqueous solution is pH 7.5 ± 0.2 at 25 °C
Cultural Response
Cultural characteristics observed after an incubation at 42°C for 24-48 hours in presence of 5% Oxygen, 10% Carbon dioxide and 85% Nitrogen.
| Organisms (ATCC) | Growth* |
|---|---|
| Campylobacter coli (33559) | luxuriant |
| Campylobacter jejuni (29428) | luxuriant |
| Escherichia coli (25922) | inhibited |
| Staphylococcus aureus (25923) | inhibited |
Key: * = > With added Campylobacter Selective Supplement IV (FD042).
References
- Bolton F.J. and Robertson L., 1982, J. Clin. Pathol., 35:462.
- Vanderzant C. and Splittstoesser D. (Eds.), 1992, Compendium of Methods for the Microbiological Examination of Foods, 3rd ed., APHA, Washington, D.C.
- Stern N.J., 1982, J. Food Safety, 4:169.
| Product Name | Preston HiVeg™ Agar Base |
|---|---|
| SKU | MV939 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Bolton F.J. and Robertson L., 1982, J. Clin. Pathol., 35:462. |
| Customized Product Available | No |
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