HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Intended Use
Letheen HiVeg Agar / Broth is recommended for determination of bactericidal activity of quaternary ammonium compounds using Escherichia coli or Staphylococcus aureus.
Composition
| Ingredients | MV414 Grams/Litre | MV165 Grams/Litre |
|---|---|---|
| HiVeg peptone | — | 10.00 |
| HiVeg hydrolysate | 5.00 | — |
| HiVeg extract | 3.00 | 5.00 |
| Dextrose | 1.00 | — |
| Polysorbate 80 | 7.00 | 5.00 |
| Sodium chloride | — | 5.00 |
| Lecithin | 1.00 | 0.70 |
| Agar | 15.00 | — |
| Final pH (at 25°C) | 7.0 ± 0.2 | |
Formula adjusted, standardized to suit performance parameters
Product Profile
Vegetable based (Code MV)
- MV414/MV165
- HiVeg peptone
- HiVeg extract
- HiVeg hydrolysate
Animal based (Code M)
- M414/M165
- Peptic digest of animal tissue
- Beef extract
- Casein enzymic hydrolysate
Recommended for
Determination of bactericidal activity of quaternary ammonium compounds using Escherichia coli or Staphylococcus aureus.
Directions
Suspend 32 grams of MV414 or 25.7 grams of MV165 in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
These media are prepared by completely replacing animal based peptones with vegetable peptones which are free from BSE/TSE risks. These media are the modification of Tryptone Glucose Extract Agar developed according to APHA (1) for use in the microbiological examination of water which was further modified by Weber and Black (2) with addition of lecithin and polysorbate 80 which resulted in a medium that effectively neutralizes quaternary ammonium compounds in the testing of germicidal activity (3). HiVeg extract, HiVeg hydrolysate or HiVeg peptone, dextrose supply nitrogenous compounds, carbon, sulphur and other trace elements to the organisms. Lecithin and polysorbate 80 enables the recovery of bacteria from solutions containing residues of disinfectant used in sanitization of utensils and equipments. Lecithin neutralizes quaternary ammonium compounds and polysorbate 80 neutralizes phenolic disinfectants, hexachlorophene and formalin (4, 5). Dehydrated medium may appear moist with 'brown sugar' appearance, does not indicate deterioration.
Reconstitution
- (MV414): 32.0 g/l
- (MV165): 25.7 g/l
Quantity on preparation (500g)
- (MV414): 15.62 L
- (MV165): 19.45 L
pH (25°C)
7.0 ± 0.2
Supplement
None
Sterilization
121°C / 15 minutes.
Storage
Dry Medium and Prepared Medium 2 - 8°C.
Quality Control
Appearance of Powder: Dark yellow coloured may have slight lumps.
Gelling: Firm, comparable with 1.5% Agar gel of MV414.
Colour and Clarity: Light yellow coloured, clear to slightly opalescent gel forms in petri plates, clear solution in tubes.
Reaction: Reaction of 3.2% w/v of MV414 or 2.57% w/v of MV165 aqueous solution is pH 7.0 ± 0.2 at 25°C.
Cultural Response: Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Escherichia coli (25922) | 102-103 | good to luxuriant | > 70% |
| Staphylococcus aureus (6538) | 102-103 | good to luxuriant | > 70% |
| Product Name | Letheen HiVeg™ Agar |
|---|---|
| SKU | MV414 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Weber and Black, 1948, Soap Sanitary Chem., 24:134. 2.Favero (Chm.), 1967, A State of the Art Report, Biological Contamination Control Committee, American Association forContamination Control. 3.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williamsand Wilkins, Baltimore4.American Society for Testing and Materials, 1991, Standard Test Methods for preservatives in water-containing cosmetics,E640-7 |
| Customized Product Available | No |
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