HiPurA® SuperPlant DNA Purification Kit (For purification of DNA from plants rich in polyphenols)

This kit simplifies isolation of DNA from fresh plant material with spin column procedure. The procedure is optimized for a maximum of 200 mg of wet‐weight of the starting material. The sample (fresh) is cut and ground in liquid nitrogen along with SuperPlant Extraction Buffer. SuperPlant Extraction Buffer contains CTAB (Cetyltrimethylammonium bromide), a detergent used to break open plant cells and solubilize the contents. Chlorophyll and some denatured proteins are removed from green plant tissue in an organic chloroform‐ isoamylalcohol step, and the organic phase is separated by centrifugation. Since the extract contains DNA and RNA, RNA can be removed by the addition of RNase A. The flow‐through fraction is then mixed with a solution that enhances the binding of DNA to the column. The solution is then passed through HiElute Miniprep Spin Column (Capped) that is followed by washing steps to remove trace contaminants. High quality DNA is eluted in the Elution Buffer (ET) provided in the kit.