HiPurA® Cotton DNA Isolation Kit

DNA extraction from plant tissues, unlike DNA isolation from mammalian tissues, remains difficult due to the presence of a rigid cell wall surrounding the plant cells. Cotton leaves contain high levels of polyphenolic compounds that irreversibly interact with proteins and nucleic acids during DNA isolation. In order to overcome the problems associated with poor quality of DNA, this kit has been developed. The kit helps to extract good quality DNA which is compatible for downstream applications. This method can be used for both on Herbarium specimens and on fresh leaves. The scale of extraction is dependent on the amount of starting material. Typical yield from 100 mg of wet weight sample is 7-12 µg.