HiPurA® Insect DNA Purification Kit

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SKU:
MB529
HiPurA® Insect DNA Purification Kit provides a fast and easy method for purification of total DNA for reliable applications in PCR, Southern blotting technique etc. The DNA purification procedure using the miniprep spin column comprises of three steps viz. adsorption of DNA to the membrane, removal of residual contaminants and elution of pure genomic DNA. HiMedia's HiElute Miniprep Spin Column (Capped) format allows rapid processing of multiple samples. The columns have a high binding capacity and high quality DNA is obtained from various species. The genomic DNA obtained is compatible with downstream applications such as restriction endonuclease digestion, PCR and Southern blotting.


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HiPurA® Insect DNA Purification Kit provides a fast and easy method for purification of total DNA from insects, arthropods, roundworms, flatworms, etc using the miniprep spin column. The method is suitable for fresh as well as frozen insect samples. Insects are ground in liquid nitrogen using a mortar and pestle and subjected to lysis by Porteinase K in salt solution, followed by adsorption of DNA to the membrane, removal of residual contaminants and elution of pure genomic DNA. adsorption of DNA to the membrane, removal of residual contaminants and elution of pure genomic DNA. Concentration of recovered DNA can be estimated by agarose gel electrophoresis. Typically 50 mg of tissue yields upto 5-15 µg of DNA.
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Product Name HiPurA® Insect DNA Purification Kit
SKU MB529
Customized Product Available No
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