HiPurA® Water DNA Purification Kit provides a fast and easy method for purification of total DNA for reliable applications in PCR, Southern blotting technique etc. The DNA purification procedure using the miniprep spin columns comprises of three steps viz, adsorption of DNA to the membrane, removal of residual contaminants and elution of pure genomic DNA. HiMedia's HiElute Miniprep Spin Column (Capped) format allows rapid processing of multiple samples. The columns have a high binding capacity and high-quality DNA is obtained from various species. The DNA obtained is compatible with downstream applications such as restriction enzyme digestion, PCR amplification and Southern blotting.
Technical Data Sheet (TD)
HiPurA® Water DNA Purification Kit (with enrichment)
Safety Data Sheet (SDS)
HiPurA® Water DNA Purification Kit (with enrichment)
Electronic Instructions for Use (eIFU)
HiPurA® Water DNA Purification Kit (with enrichment)
HiPurA® Water DNA Purification Kit (with enrichment)
HiPurA® Water DNA Purification Kit provides a fast and easy method for purification of total DNA using the miniprep spin columns. It is is based on the advanced silica binding principle presented in a microspin format. It comprises of three steps viz, adsorption of DNA to the membrane, removal of residual contaminants and elution of pure genomic DNA. It is a convenient method for detection of microorganisms from environmental water samples allowing rapid isolation and purification of total DNA from bacteria and fungi found in water samples. The water sample is passed through a 0.22µm filter and the microorganisms present in the water are trapped and enriched for purification. Concentration of DNA can be estimated by agarose gel electrophoresis.