HiPurA® Soil DNA Purification Kit provides a fast and easy method for purification of total DNA from environmental samples containing a high humic acid content including difficult soil types such as compost, sediment, manure and other common soil types, for reliable applications in PCR and other downstream applications. The HiPurA® DNA purification system combines the reversible nucleic acid binding properties of HiElute Miniprep Spin Column (Capped) with an efficient buffer system, which eliminates PCR inhibiting compounds such as humic acid from soil samples.
HiPurA® Soil DNA Purification Kit provides a fast and easy method for purification of total DNA from environmental samples containing a high humic acid content including difficult soil types such as compost, sediment, manure and other common soil types using the miniprep spin column. It is is based on the advanced silica binding principle presented in a microspin format facilitating the binding, washing and elution steps thus enabling multiple samples to be processed simultaneously. In this kit the soil sample is lysed and homogenized in a bead-beating step. Concentration of DNA can be estimated by agarose gel electrophoresis. Typical yield is about 20µg from 250 - 500mg soil.