HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Bi.G.G.Y HiCynth™ Agar (Nickerson HiCynth™ Medium)
For detection, selective isolation, differentiation and presumptive identification of Candida albicans and Candida tropicalis.
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Intended Use
Recommended for detection, selective isolation, differentiation and presumptive identification of Candida albicans and Candida tropicalis from various samples.
Composition
| Ingredients | Gms / Litre |
|---|---|
| HiCynth™ Peptone No.3* | 1.000 |
| Glycine | 10.000 |
| Dextrose (Glucose) | 10.000 |
| Ammonium Bismuth Citrate | 5.000 |
| Sodium sulphite | 3.000 |
| Agar | 16.000 |
| Final pH (at 25°C) | 6.8±0.2 |
**Formula adjusted, standardized to suit performance parameters
*Chemically defined peptone
Directions
Suspend 45 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE OR OVERHEAT. Overheating will destroy the selective properties. Cool to 45-50°C. Disperse the flocculant precipitate formed by swirling prior to dispensing into Petri plates.
Principle And Interpretation
In a study of sulphite reduction by yeasts, the ability of many types of yeast to reduce bismuth sulphite was noted. Growth on an acidic or neutral medium containing bismuth sulphite produced black colonies because of the extra cellular reaction of the bismuth sulphite to bismuth sulphide.
Bi.GGY. Agar (Nickerson Agar) was originally formulated by Nickerson (1,2) and further modified by Haley (3) following study of sulphite reduction. This medium is only a part of the identification process of organisms. Other tests may be required.
Bi.G.G.Y HiCynth™ Agar is prepared by replacing animal and vegetable peptones with chemically defined peptones to avoid BSE/TSE risks associated with animal peptones. Bi.G.G.Y HiCynth™ Agar can also be tested for clinical specimens such as tissues, skin scrapings, hair, nail clipping etc. (4, 5). HiCynth™ Peptone No.3, dextrose and glycine serve as nutrients.. Bismuth ammonium citrate and sodium sulphite together act as selective agents for Candida species suppressing bacterial growth, at the same time indicating substrate reduction to yield bismuth sulphite which helps to presumptively identify Candida species. Precipitate present in molten medium should be uniformly suspended while plating the agar so do not use slants of medium.. This medium may be used for the isolation and presumptive identification of C. albicans and C. tropicalis from sputum (3) and vaginal smears (6).
Type of specimen
Food and dairy samples
Specimen Collection and Handling
For food and dairy samples follow appropriate techniques for handling specimens as per established guidelines (7,8). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Some species of Candida may show poor growth due to nutritional variations.
- Further biochemical and serological tests must be carried out for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.6% Agar gel.
Colour and Clarity of prepared medium
Light amber coloured, opalescent gel (with a dispersible flocculant precipitate) forms in Petriplates
Reaction
Reaction of 4.5% w/v aqueous solution at 25°C. pH: 6.8±0.2
pH
6.60-7.00
Cultural Response
Cultural characteristics observed after an incubation at 25-30°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Colony morphology |
|---|---|---|---|---|
| Candida albicans ATCC 10231 (00054*) | 50-100 | luxuriant | >=50% | smooth, circular intensly brown black, no colour diffusion and no sheen |
| Candida kruisei ATCC 24408 | 50-100 | luxuriant | >=50% | large flat, wrinkled silvery brown, black colonies with brown peripheries, yellow halo |
| Candida tropicalis ATCC 750 | 50-100 | luxuriant | >=50% | smooth discrete, dark brown with black centres, diffused blackening after 72 hours, sheen, slight mycelial fringe |
| Escherichia coli ATCC 25922 (00013*) | >=104 | inhibited | 0% | |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | >=104 | inhibited | 0% | |
| Candida pseudotropicalis | 50-100 | Good | 40-50% | Dark reddish brown, glistening colony |
Key : (*)Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (10,11).
| Product Name | Bi.G.G.Y HiCynth™ Agar (Nickerson HiCynth™ Medium) |
|---|---|
| SKU | MCD217 |
| Product Type | HiCynth™ |
| Physical Form | Powder |
| Origin | Chemically defined (HiCynth™) |
| Packaging type | HDPE |
| References | 1. Nickerson W.J., 1947, The Chronica, Botanica Co. 2.Nickerson W.J., 1953, J. Inf. Dis., 93:43. 3.Haley L.D., 1959, Trans. N.Y. Acad. Sci., 21(8):708. 4.Lennette, Balows, Hausler and Shadomy (Eds.), 1985, Manual of Clinical Microbiology, 4th ed., A.S.M. Washington, D.C. 5.MacFaddin J.F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. I, Williamsand Wilkins, Baltimore.. 6.Mendel E.B., Naberman S. and Hall D. K., 1960, Obstel and Gynec.16, 180-184. |
| Customized Product Available | No |
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