HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Composition**
| Ingredients | Gms / Litre |
|---|---|
| HiCynth™ Peptone No.1 | 15.000 |
| HiCynth™ Peptone No.5 | 3.000 |
| Lactose | 12.500 |
| Synthetic Detergent I | 1.500 |
| Sodium chloride | 5.000 |
| Aniline blue | 0.100 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.4±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 52.1 grams in 1000 ml distilled water containing 10 ml 1% Rosolic Acid (FD058). Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
M-FC Agar Base, designed by Geldreich et al (2) is used for the detection and enumeration of faecal coliforms using the membrane filter technique. This medium is based on the property of faecal coliforms to grow at 44-45°C (1). M-FC Agar Base is recommended by APHA (3) and by various other standards for detection of faecal coliforms (4-6). APHA recommends the membrane filtration procedure and delayed incubation for faecal coliforms. M-FC HiCynth™ Agar Base is the modification of the same using chemically defined peptones instead of animal peptones to avoid BSE/TSE risk.
HiCynth™ Peptone No.1 and HiCynth™ Peptone No.5 provides carbon, nitrogen compounds, long chain amino acids, vitamins and other essential nutrients for the growth of faecal coliforms. Lactose is the carbon source as well as fermentable carbohydrate in the medium. Synthetic detergent I inhibit the growth of contaminating gram-positive microorganisms. Aniline blue is a triphenyl methane dye which suppresses the growth of many gram-positive microorganisms. Aniline blue along with rosolic acid forms the indicator system of the medium.
Membrane filters, through which water sample is passed are aseptically placed onto M-FC HiCynth™ Agar base plates. If total coliforms are to be estimated, incubation is carried out at 35-37°C whereas if faecal coliform count is to be estimated, incubation is done at 44-45°C. Coliforms will form blue colonies whereas non-coliforms will form gray coloured colonies on M-FC Agar Base.
Quality Control
Appearance
Light yellow to greyish yellow, may have slight green or blue tinge homogeneous free
flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
After Addition of 1% Rosolic Acid: Red coloured slightly
opalescent gel forms in Petri plates
Reaction
Reaction of 5.21% w/v aqueous solution at 25°C. pH : 7.4±0.2
pH
7.20-7.60
Cultural Response
Cultural characteristics observed with added 1% Rosolic Acid (FD058) after an incubation at different temperatures for 22-24 hours.
| Organism | Inoculum (CFU) | Growth at 35-37°C | Growth at 45.5°C | Colour of colony (on membrane filter) |
|---|---|---|---|---|
| Enterococcus faecalis ATCC >=103 29212 | inhibited | inhibited | ||
| Escherichia coli ATCC 25922 | 50-100 | luxuriant | luxuriant | light blue |
| Salmonella Typhimurium ATCC 14028 | 50-100 | luxuriant | inhibited | pale pink - pink |
| Shigella flexneri ATCC 12022 | 50-100 | luxuriant | inhibited | pinkish |
Storage and Shelf Life
Store below 30°C in a tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Use before expiry date on the label. Product performance is best if used within stated expiry period.
| Product Name | M-FC HiCynth™ Agar Base |
|---|---|
| SKU | MCD1122 |
| Product Type | HiCynth™ |
| Physical Form | Powder |
| Origin | Chemically defined (HiCynth™) |
| Packaging type | HDPE |
| References | 1. Collee J. G., Fraser A. G., Marmion B. P., Simmons A., (Eds.) Mackie and McCartney, Practical Medical Microbiology,1996, 14th Edition, Churchill Livingstone. |
| Customized Product Available | No |
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