HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg hydrolysate No. 1 | 10.0 |
| HiVeg peptone No. 3 | 5.0 |
| Yeast extract | 3.0 |
| Sodium chloride | 5.0 |
| Inositol | 10.0 |
| Synthetic detergent No. I | 1.5 |
| Aniline blue | 0.1 |
| Agar | 15.0 |
Final pH (at 25°C) 7.4 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 49.6 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Add 10 ml of 1% Rosolic Acid (FD058). Cool below 45°C and add 50 mg Carbenicillin. Mix well and pour into sterile petri plates.
Principle and Interpretation
This medium is prepared by using vegetable peptones that are free of BSE/TSE risks associated with animal based peptones. M-FC Agar Base, Modified is formulated as per APHA (1) for enumeration of Klebsiella. In this medium M-FC Agar is modified by replacing lactose with inositol and addition of Carbenicillin. M-FC HiVeg Agar Base, Modified is the modification of M-FC Agar Base Modified. Sample volume is selected to yield 20 to 60 Klebsiella colonies per membrane. This membrane filter is placed on agar surface. Occasional false positive occurrences are caused by Enterobacter species. Klebsiella colonies appear deep blue to blue grey due to the aniline blue present in the medium. HiVeg hydrolysate No. 1, HiVeg peptone No.3, yeast extract provides nitrogenous compounds, sulphur, vitamins and other nutrients. Inositol is the fermentable carbohydrate. Synthetic detergent No. I inhibits gram-positive organisms. Carbenicillin acts as an inhibitory substance.
Quality Control
Appearance of powder
Yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Product Profile
| Vegetable based (Code MV) MV1124 | Animal based (Code M) M1124 |
|---|---|
| HiVeg hydrolysate No. 1 | Tryptose |
| HiVeg peptone No. 3 | Proteose peptone |
| Synthetic detergent No. I | Bile salts mixture |
Recommended for
Rapid enumeration of Klebsiella using membrane filter technique
Reconstitution
49.6 g/l
Quantity on preparation (500g)
10.08 L
pH (25°C)
7.4 ± 0.2
Supplement
- Rosolic Acid (FD058)
- Carbenicillin
Sterilization
Boiling (DO NOT AUTOCLAVE).
Storage
Dry Medium - Below 30°C, Use freshly prepared medium.
Gelling
Firm, comparable with 1.5% Agar gel.
Colour and Clarity
With addition of 1% Rosolic Acid (FD058), red coloured slightly opalescent gel forms in petri plates.
Reaction
Reaction of 4.96% w/v aqueous solution is pH 7.4 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 24 hours with added Rosolic Acid (FD058) and Carbenicillin.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Colour of colony* |
|---|---|---|---|
| Enterobacter aerogenes (13048) | 20-60 | good-luxuriant | pink or pale yellow |
| Klebsiella pneumoniae (13883) | 20-60 | good-luxuriant | deep blue-blue grey |
Key : * = on membrane filter
References
- Greenberg A. E., Trussell L. S. (ed.), 1985, Standard methods R. R. and Clesceri for the examination of water and waste water, 16th ed., APHA, Washington, D.C.
| Product Name | M-FC HiVeg™ Agar Base, Modified |
|---|---|
| SKU | MV1124 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1.Eaton A. D., Clesceri L. S. and Greenberg A. W., (Eds.), 2005, Standard Methods for the Examination of Water andWastewater, 21st Ed., APHA, Washington, D.C. |
| Customized Product Available | No |
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