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Soyabean Casein Digest Medium (Tryptone Soya Broth)

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SKU:

M011

Recommended as a general purpose medium used for cultivation of a wide variety of microorganisms and recommended for sterility testing of moulds and lower bacteria.

Description

Intended Use

Recommended as a general purpose medium used for cultivation of a wide variety of microorganisms and recommended for sterility testing of moulds and lower bacteria.

Composition

Ingredients	g / L
Tryptone	17.000
Soya peptone	3.000
Sodium chloride	5.000
Dextrose (Glucose)	2.500
Dipotassium hydrogen phosphate	2.500

Final pH (at 25°C): 7.3±0.2

Formula adjusted, standardized to suit performance parameters.

Directions

Suspend 30.0 grams in 1000 ml purified/distilled water. Heat if necessary to dissolve completely. Mix well and dispense as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

If any fibres are observed, filter the solution through a 0.22 µm filter to eliminate them.

Principle and Interpretation

Soyabean Casein Digest Medium is recommended by various pharmacopeias for sterility and microbial limit testing. It is highly nutritious, supporting a wide range of organisms. Tryptone and soya peptone supply nitrogen, amino acids, and peptides; glucose provides carbohydrate; phosphate buffers the medium; sodium chloride maintains osmotic balance.

Type of Specimen

Pharmaceutical samples; clinical samples such as urine, pus, and wound exudates.

Specimen Collection and Handling

For clinical samples, follow standard handling techniques per established guidelines. For pharmaceutical samples, adhere to pharmacopeial procedures. Contaminated materials must be sterilized by autoclaving before disposal.

Warning and Precautions

For In Vitro diagnostic use. Professional use only. Read label before opening. Use PPE. Follow standard microbiological practices and safety protocols.

Limitations

Further biochemical characterization of pure colonies is required for identification.
This general-purpose medium may not support growth of fastidious organisms.

Performance and Evaluation

Expected performance is achieved when used per instructions and within expiry period at recommended storage conditions.

Quality Control

Appearance: Cream to yellow homogeneous free flowing powder.

Colour and Clarity: Light yellow clear solution without precipitate.

Reaction: pH 7.3 ± 0.2 (3% w/v aqueous solution at 25°C after sterilization).

Stability Test: Clear yellow solution stable for 7 days at room temperature.

Growth Promoting Properties

Visible growth comparable with approved control lot within specified incubation conditions per USP/EP/BP/JP/IP.

Organism	Inoculum (CFU)	Growth	Temperature	Incubation Period
Salmonella Typhimurium ATCC 14028	50–100	Luxuriant	30–35°C	18–24 hrs
Salmonella Abony NCTC 6017	50–100	Luxuriant	30–35°C	18–24 hrs
Pseudomonas paraeruginosa ATCC 9027	50–100	Luxuriant	30–35°C	18–24 hrs
Staphylococcus aureus subsp. aureus ATCC 6538	50–100	Luxuriant	30–35°C	18–24 hrs
Escherichia coli ATCC 25922	50–100	Luxuriant	30–35°C	18–24 hrs
Escherichia coli ATCC 8739	50–100	Luxuriant	30–35°C	18–24 hrs
Bacillus spizizenii ATCC 6633	50–100	Luxuriant	30–35°C	18–24 hrs
Kocuria rhizophila ATCC 9341	50–100	Luxuriant	30–35°C	18–24 hrs
Pseudomonas aeruginosa ATCC 27853	50–100	Luxuriant	30–35°C	18–24 hrs
Candida albicans ATCC 10231	50–100	Luxuriant	20–25°C	≤5 days
Staphylococcus aureus subsp. aureus ATCC 25923	50–100	Luxuriant	30–35°C	18–24 hrs
Aspergillus brasiliensis ATCC 16404	50–100	Luxuriant	20–25°C	≤5 days
Candida albicans ATCC 2091	50–100	Luxuriant	20–25°C	≤5 days

*Corresponding WDCM numbers. ^Formerly known as Pseudomonas aeruginosa. #Formerly known as Aspergillus niger. **Formerly known as Bacillus subtilis subsp. spizizenii. $Formerly known as Micrococcus luteus.

Storage and Shelf Life

Store between 10–30°C in tightly closed container. Prepared medium: 15–30°C. Protect from moisture and ignition sources. Seal tightly after use. Best used within stated expiry period.

Disposal

Dispose by autoclaving and/or incineration of used media. Follow biosafety and laboratory protocols for infectious waste handling.

References

Indian Pharmacopoeia, 2022.
MacFaddin J. F., 1985. Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria.
United States Pharmacopoeia-National Formulary (USP-NF), 2022.
Forbes B.A., Sahm D.F., Weissfeld A.S. (1998). Bailey & Scott’s Diagnostic Microbiology.
Isenberg H.D., Clinical Microbiology Procedures Handbook, 2nd Ed.
Jorgensen J.H. et al., 2015. Manual of Clinical Microbiology, 11th Ed.

Product Information

More Information
Product Name	Soyabean Casein Digest Medium (Tryptone Soya Broth)
SKU	M011
Product Type	Regular
Physical Form	Powder
Origin	Animal
Packaging type	HDPE
References	1. MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams& Wilkins, Baltimore, M.d. 2.The United States Pharmacopeia, 2018, USP 41/NF36, The United States Pharmacopeial Convention, Rockville, MD. 3.Indian Pharmacopeia, 2018, Govt. of India, Ministry of Health and Family Welfare, New Delhi, India. 4.Forbes B. A., Sahm D. F. and Weissfeld A. S., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc. St.Louis, Mo. 5.Isenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition. 6.Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)Manual of Clinical Microbiology, 11th Edition. Vol. 1.
Customized Product Available	No