HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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TSB - Tryptone Soya Broth Supplemented w/ 0.05% SPS
This medium is recommended as a qualitative test for detection of microorganisms in blood . Sodium polyanetholesulfonate (SPS), an anticoagulant which inhibits the antimicrobial systems of blood, is used widely in blood culture medium and cause an increase in recovery of these organisms. It contains 20 ml medium and is used for paediatric use .
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Intended Use
Recommended as a qualitative test for detection of microorganisms in blood. Sterile, in glass bottles.
Composition
| Ingredients | g/L |
|---|---|
| Tryptone | 17.000 |
| Soya peptone | 3.000 |
| Sodium chloride | 5.000 |
| Dextrose (Glucose) | 2.500 |
| Dipotassium hydrogen phosphate | 2.500 |
| Sodium polyanethol sulphonate (SPS) | 0.500 |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Label the ready to use blood culture bottle. Do not unscrew cap. remove the top of the screw cap. Disinfect the part of the rubber stopper which is now exposed. Draw patient's blood with the sterile or disposable needle and syringe as explained in specimen collection and disposable column. Transfer the blood sample immediately into the culture bottle by puncturing the rubber stopper with the needle and injecting the blood. Venting: Use sterile venting needle (LA038). Keep the bottle in an upright position preferably in a biological safety cabinet, place an alcohol swab over the rubber stopper and insert the venting needle with filter through it. Insertion and withdrawal of the needle should be done in a straight line. discard the needle and mix the contents by gently inverting the bottle 2-3 times. Do Not vent the bottle for anaerobic cultures. Incubate at 35±2°C for 18-24 hours and further for seven days.
Principle And Interpretation
Soyabean Casein Digest Medium is recommended by various pharmacopeias as a sterility testing and as a microbial limit testing medium (1,2). This medium is a highly nutritious medium used for cultivation of a wide variety of organisms (3,4). Bacteremia is a serious and often life-threatening clinical condition. An important diagnostic tool for this condition is to analyze a blood specimen for the growth of bacteria on selected growth media. Such media often contain SPS as an anticoagulant and as an inhibitor of the bacteriostatic and bactericidal effects of blood cells and plasma factors (5,6). The combination of tryptone and soya peptone makes the medium nutritious by providing amino acids and long chain peptides for the growth of microorganisms. Dextrose (Glucose) and dipotassium hydrogen phosphate serve as the carbohydrate source and the buffer, respectively in the medium. Sodium chloride maintains the osmotic balance of the medium. Sodium Polyanethole Sulphonate (SPS) which is an anticoagulant in culture bottles promotes optimal recovery of organisms from blood (7). It acts to inhibit phagocytosis and to neutralize the antibacterial activity of fresh blood components (8,9).
Type of specimen
Clinical sample: Blood
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (10,11). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile clear Tryptone Soya Broth supplemented w/ 0.05% SPS in glass bottle.
Colour
Light yellow coloured clear solution
Quantity of Medium
20 ml of medium in glass bottle, (Volume of blood for paediatrics use - 1 to 3 ml)
pH
7.10-7.50
Sterility Check
Passes release criteria.
Cultural response
Cultural characteristics observed after an incubation at at 35-37°C for <= 3 days
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | luxuriant |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant |
| Pseudomonas aeruginosa ATCC 27853 (00025*) ^ | 50-100 | luxuriant |
| Bacillus spizizenii ATCC 6633 (00003*) | 50-100 | luxuriant |
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | luxuriant |
| Salmonella Abony NCTC 6017 (00029*) | 50-100 | luxuriant |
| Streptococcus pneumoniae ATCC 6305 | 50-100 | luxuriant |
| Streptococcus pyogenes ATCC 19615 | 50-100 | luxuriant |
| Candida albicans ATCC 10231 (00054*) | 50-100 | luxuriant |
| # Aspergillus brasiliensis ATCC 16404 (00053*) | 50-100 | luxuriant |
Key : (*) Corresponding WDCM numbers, (#) Formerly known as Aspergillus niger
^ Formerly known as Bacillus subtilis subsp. spizizenii
Storage and Shelf Life
On receipt store between 15-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (10,11).
| Product Name | TSB - Tryptone Soya Broth Supplemented w/ 0.05% SPS |
|---|---|
| SKU | LQ011 |
| Customized Product Available | No |
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