HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Super Broth No. II, granulated is used for the cultivation of recombinant strains of Escherichia coli.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Tryptone | 12.000 |
| Yeast extract | 24.000 |
| Dipotassium phosphate | 11.400 |
| Monopotassium phosphate | 1.700 |
| Final pH (at 25°C) | 7.2±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 49.1 grams in 1000 ml distilled water containing 5 ml glycerol. Heat if necessary to dissolve the medium completely.
Dispense in tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 mins.
Principle And Interpretation
The rearrangement of genetic information within and among DNA molecule encompasses a variety of processes, collectively placed under the heading of genetic recombination (1). Bacteria that have undergone recombination, need to be grown in an enriched medium. Super Broth No. II is a modification of Super Broth, developed by Tartoff and Hobbs (2).
High amount of tryptone and yeast extract, make the medium highly nutritive for the growth of recombinant strains of E.coli. Phosphate provide buffering to the medium.
Quality Control
Appearance
Cream to yellow coloured granular medium
Colour and Clarity of prepared medium
Amber coloured clear solution without any precipitate
Reaction
Reaction of 4.9% w/v aqueous solution at 25°C. pH: 7.2±0.2
pH
7.00-7.40
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Escherichia coli ATCC 25922 | 50-100 | good-luxuriant |
| Escherichia coli ATCC 23724 | 50-100 | good-luxuriant |
| Staphylococcus aureus ATCC 25923 | 50-100 | good-luxuriant |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.
| Product Name | Super Broth No.II, Granulated |
|---|---|
| SKU | GM1689 |
| Product Type | Granulated |
| Physical Form | Granular |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Nelson D.L, Cox M.M, 2005 Lehninger Principles of Biochemistry, 4th edi, W.H. Freeman and Company. New York.2.Tartoff and Hobbs . 198 |
| Customized Product Available | No |
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