HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Intended Use
Recommended for mass cultivation of Escherichia coli.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg hydrolysate | 35.0 |
| Yeast extract | 20.0 |
| Sodium chloride | 5.0 |
** Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 60.0 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
This medium is prepared by using HiVeg hydrolysate in place of Casein enzymic hydrolysate which makes the medium free of BSE/TSE risks. Super HiVeg Broth is modification of the formulation cited in Atlas (1) and it is used for mass cultivation of Escherichia coli. HiVeg hydrolysate and yeast extract provide nitrogenous compounds, Vitamin B-Complex and other essential growth nutrients. Sodium chloride maintains osmotic equilibrium. Super HiVeg Broth is nutritionally rich hence other organisms can also grow in it easily.
Product Specifications
Reconstitution: 60.0 g/l
Quantity on preparation (500g): 8.33 L
pH (25°C): -
Supplement: None
Sterilization: 121°C / 15 minutes.
Storage: Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Quality Control
Appearance of powder: Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Colour and Clarity: Light yellow coloured, clear without any precipitate.
Cultural Response: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth |
|---|---|---|
| Escherichia coli (23724) | 102-103 | good-luxurinat |
| Escherichia coli (25922) | 102-103 | good-luxuriant |
| Staphylococcus aureus (25923) | 102-103 | good-luxuriant |
References
- Atlas R.M., 1993, Handbook of Microbiological Media, Parks L.C. (Ed.), CRC Press, Inc.Use before expiry date on the label.
| Product Name | Super HiVeg™ Broth |
|---|---|
| SKU | MV1316 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Atlas R.M., 2004, Handbook of Microbiological Media, Parks L.C. (Ed.), CRC Press, Inc |
| Customized Product Available | No |
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