Minimum Essential Medium Eagle (MEM) w/ Earle’s salts, NEAA, Sodium bicarbonate and L-Glutamine Hybridoma Tested

Minimum Essential Medium(MEM) is a modification of Basal Medium Eagle(BME). It was developed by Harry Eagle to meet the specific nutritional requirements of certain subtypes of HeLa cells and normal mammalian fibroblasts. MEM includes higher concentration of amino acids so as to closely approximate the protein composition of cultured mammalian cells. By eliminating calcium, it can facilitate growth of cells in suspension cultures. AL047H is hybridoma tested medium containing Earle's salts, L-glutamine, sodium bicarbonate and non-essential amino acids.