Mitsuhashi and Maramorosch Insect Medium w/ Sodium bicarbonate

Mitsuhashi and Maramorosch Insect Medium is specially used for the growth and propagation of mosquito cell lines especially Aedes aegypticus. It was originally developed to grow cells derived from Leafhopper. Subsequently, it has been used to culture cells derived from a number of insect species. IML002 needs to be supplemented with 5-20% fetal bovine serum. Lactalbumin hydrolysate serves as a source of free amino acids, whereas yeast extract serves as a source of vitamins. When supplemented with fetal bovine serum, this medium is most commonly used to culture cells derived from a number of mosquitoes.