Earle's Balanced Salt Solution 1X w/ Sodium bicarbonate and Phenol red w/o Calcium and Magnesium

All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt solution to be developed specifically for supporting the metabolism of mammalian cells was Tyrode's solution. Since then many modifications have been done to obtain better buffering salt solutions and to prevent calcium precipitation. Earle's balanced salt solution is designed to equilibrate with a 5% CO2 in air mixture. Earle's balanced salt solution contains sodium bicarbonate and phenol red hence requires the cells to be grown in a 5% CO2 environment. It does not contain calcium and magnesium.