Blasticidin S Hydrochloride, Cell Culture Tested

Blasticidin S Hydrochloride is an antibiotic that belong to the cytosine amino nucleoside antibiotic group. It is isolated from the bacterial culture broth of Streptomyces griseochromogenes. It inhibits protein synthesis in eukaryotic as well as prokaryotic cells by inhibiting peptide bond formation. Blasticidin resistance is conferred by the Blasticidin deaminase resistance gene from Bacillus cereus (bsr) or from Aspergillus terreus. During cell growth this antibiotic gets converted to the nontoxic deaminohydroxy derivative by converting Blasticidin S to a non-toxic form deaminohydroxy derivative. It also has antifungal property. In cell culture applications, Blasticidin induces rapid cell dissociation and death in several cell lines. It is used to select transfected cells carrying bsr or BSD resistance genes.

Directions:

For cell culture applications, Blasticidin S Hydrochloride solutions can be prepared as per required concentrations in water. Solutions can be sterilized by filtering through a sterile membrane filter with a porosity of 0.22 microns or less.