Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Tetracycline hydrochloride, Cell Culture Tested
SKU:
TC036
CAS Number :
64-75-5
Synonym :
Anhydrotetracycline, Abramycin, Biocycline, Cefracycline
Molecular Formula :
C₂₂H₂₄N₂O₈·HCl
Availability: In stock
Only %1 left
As low as
$35.20
Product Identifier
| Product Code | TC036 |
|---|---|
| Molecular Weight | 480.90 |
| Molecular Formula | C₂₂H₂₄N₂O₈ HCl |
| CAS Number | 64-75-5 |
| Synonym | Anhydrotetracycline, Abramycin, Biocycline, Cefracycline |
Technical Specification
| Product Description | Tetracycline is a polyketide antibiotic produced by Streptomyces spp. It inhibits protein synthesis by binding to 30S ribosomal subunit and blocking attachment of charged aminoacyl tRNA to ribosome. This prevents incorporation of new amino acids to the nascent peptide chain. It acts as a broad spectrum antibiotic against Gram-positive and Gram-negative bacteria. In cell culture media, Tetracycline finds application as an anti-bacterial agent to prevent contamination. It is also used as a selection agent for cells containing tetracycline resistance gene. Besides this, Tetracycline-based externally regulated (Tet-based) systems have been successfully used to control the expression of numerous transgenes in cultured cells. These systems work on principle of reversible switch-on-switch-off of the experimental gene in presence of Tetracycline. These are tightly regulated and highly responsive systems that produce on-demand robust expression of gene of interest in target cells. During such Tet-system based mammalian cell culture studies, an animal-free media is to be used for cell growth and to prevent any interference with excess tetracycline present in media. |
|---|---|
| Appearance | Light yellow to orange crystals or powder or solid. |
| Solubility | 33.3 mg soluble in 1 ml of water on heating. |
| pH | 1.80 - 2.80 |
| FTIR | Matches with standard pattern |
| Specific rotation | -255° to -240° (c=0.50% in 0.1M HCl at 20°C) |
| Antibiotic sensitivity | Complies |
| Potency | NLT 900 µg/mg |
| Assay (NT/HPLC/NaOH Titration) | 90.00 - 102.00% |
| Cell Culture Test | Passes |
| Preparation instructions | For cell culture applications, Tetracycline hydrochloride solution can be prepared as per required concentrations in water. |
| Sterilization instructions | Solutions can be sterilized by filtering through a sterile membrane filter with a porosity of 0.22 microns or less. |
| Storage conditions | Store powder at 2 - 8°C in air tight containers and away from bright light. |
| Stock solution storage | Stock solutions should be stored at -20°C. |
| Shelf life | 36 months. |
| Use before expiry | Use before expiry date given on the product label. |
CAS Number :
64-75-5
Synonym :
Anhydrotetracycline, Abramycin, Biocycline, Cefracycline
Molecular Formula :
C₂₂H₂₄N₂O₈·HCl
| Product Name | Tetracycline hydrochloride, Cell Culture Tested |
|---|---|
| SKU | TC036 |
| CAS No | 64-75-5 |
| Molecular formula | C₂₂H₂₄N₂O₈·HCl |
| Molecular Weight | 480.9 |
| Synonym | Anhydrotetracycline, Abramycin, Biocycline, Cefracycline |
| Storage | Store powder at 2 - 8°C in air tight containers and away from bright light. |
| Shelf Life | 3 Years |
| Appearance | Light yellow to orange crystals or powder or solid |
|---|---|
| Solubility | 33.3 mg soluble in 1 ml of water on heating |
| pH | 1.80 - 2.80 |
| FTIR | Matches with standard pattern |
| Specific Rotation | -255° to -240° (c = 0.50% in 0.1M HCl at 20°C) |
| Antibiotic Sensitivity | Complies |
| Potency | NLT 900 μg/mg |
| Assay (NT/HPLC/NaOH Titration) | 90.00 - 102.00% |
| Cell Culture Test | Passes |
| UN No. | 3077 |
|---|---|
| Class | 9 |
| Packing Group | III |
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