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Gentamicin sulphate

Name: Gentamicin sulphate
Brand: HiMedia Laboratories
SKU: TC026
Availability: InStock

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TC026

CAS Number : 1405-41-0

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Description

Product Identifier

Product Name	Gentamicin Sulphate Cell Culture Tested
Product Code	TC026

Technical Specification

Molecular Weight	C₁: 477.6, C₂: 463.6, C₁a: 449.5
Molecular Formula	C₁: C₂₁H₄₃N₅O₇, C₂: C₂₀H₄₁N₅O₇, C₁a: C₁₉H₃₉N₅O₇
CAS number	1405-41-0
Description	Gentamicin is a broad spectrum bacteriocidal agent of aminoglycoside group and is effective against gram positive bacteria and gram negative bacteria. It is a mixture of the three major components C1, C1a and C2. The ratio of the three components is as follows: C₁ - 25 - 50%, C₂-25-55%, C₁a-10-35%. Gentamicin binds to four nucleotides of 16S rRNA and a single amino acid of protein S12. This interferes with decoding site in the vicinity of nucleotide 1400 in 16S rRNA of 30S subunit. This region interacts with the wobble base in the anticodon of tRNA. This leads to interference with the initiation complex, misreading of mRNA so incorrect amino acids are inserted into the polypeptide leading to nonfunctional or toxic peptides and the breakup of polysomes into nonfunctional monosomes. In cell culture, gentamicin sulfate is used as an anti-bacterial agent to prevent contamination.
Cell culture application concentration	50mg/L
Preparation instructions	Gentamicin sulfate is soluble in water (50mg/ml). Gentamicin solution should be sterilized by filtering through a sterile membrane filter with a porosity of 0.22micron or less.
Appearance	White to off white powder
Solubility	Clear, faint yellow solution at 5gm in 100ml of water
pH of 5% solution in water	3.50-5.50
Specific rotation [alpha]20/D	+107° to +121°
Residue on ignition	NMT 1%
Potency	NLT 590IU/mg
Antibiotic sensitivity	Passes
Cell Culture Test	Passes
Storage	Store at gentamicin sulfate powder and solution at 2-8°C.
Gentamicin solution stability	stable for up to 5 days at 37°C.
Shelf Life	36 months
Use before	expiry date given on the product label.