Restriction enzymes are essential tools in molecular biology because each recognizes a unique DNA sequence and precisely cuts the double-stranded DNA molecule into smaller, distinct fragments.
Sph I is a Restriction Endonuclease purified from an m Streptomyces phaeochromogenes strain that carries the Sph I gene.
The restriction enzyme Sph I recognizes the DNA sequence G C A T G^C and optimally cleaves the DNA at 37°C when used with 10X Assay Buffer