Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Violet Red Bile Glucose Agar
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$49.50
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SKU:
SMH581
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Intended use
Recommended for detection and enumeration of Enterobacteriaceae from pharmaceutical products in accordance with the microbial limit testing by harmonized methodology of USP/EP/BP/JP/IP .
Composition
| Ingredients | g/L |
|---|---|
| Yeast extract | 3.000 |
| Gelatin peptone # | 7.000 |
| Bile salts | 1.500 |
| Sodium chloride | 5.000 |
| Glucose monohydrate | 10.000 |
| Agar | 15.000 |
| Neutral red | 0.030 |
| Crystal violet | 0.002 |
| pH after heating (at 25°C) | 7.4±0.2 |
**Formula adjusted, standardized to suit performance parameters
# Pancreatic digest of gelatin
Directions
Violet Red Bile Glucose Agar is a ready to use solid media in glass bottle. The medium is pre-sterilized, hence it does not need sterilization. Medium in the bottle can be melted either by using a pre-heated water bath or any other method. Slightly loosen the cap before melting. When complete melting of medium is observed dispense the medium in tubes as butts /slants or in plates as desired and allow to solidify. If on plate, either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically.
Principle And Interpretation
Violet Red Bile Glucose Agar is a selective medium recommended for detection and enumeration of Enterobacteriaceae especially the bile tolerant gram negative bacteria in accordance with the microbial limit testing by harmonized methodology of USP/EP/BP/JP/IP (1-5) from non-sterile products and pharmaceutical preparations.
Gelatin peptone and yeast extract provide nitrogenous, carbonaceous compounds, long chain amino acids, vitamins and other nutrients essential for bacterial metabolism. This media is selective due to presence of the inhibitors; bile salts positive organisms especially Staphylococci. Neutral red indicator helps to detect glucose fermentation. Glucose fermenting and crystal violet. Crystal violet inhibits gram-strains produce red colonies with pink-red halos in the presence of neutral red. Sodium chloride maintains the osmotic equilibrium in the medium. The red colour is due to absorption of neutral red and a subsequent colour change of the dye when the pH of medium falls below 6.8.
Type of specimen
Pharmaceutical samples.
Specimen Collection and Handling
For pharmaceutical samples, follow appropriate techniques for sample collection, processing as per guidelines (1-5). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions:
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Though the medium is for selective isolation of Enterobacteriaceae, further biochemical and serological testing must be carried out for further confirmation.
- Over incubation may result in reverting of reaction.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Violet Red Bile Glucose Agar in bottles
Colour of medium
Reddish purple coloured medium.
Quantity of medium
100ml of medium in bottles
pH
7.20-7.60
Sterility Check
Passes release criteria
Growth Promotion Test
Growth Promotion is carried out in accordance with the harmonized method of USP/EP/BP/JP. Cultural response was observed after an incubation at 30-35°C for 18-24 hours. Recovery rate is considered as 100% for bacteria growth on Soyabean Casein Digest Agar.
Growth promoting properties
Growth of microorganism comparable to that previously obtained with previously tested and approved lot of medium occurs at the specified temperature for not more than the shortest period of time specified inoculating <=100 cfu (at 30-35°C for <=18 hours).
Indicative properties
Colonies are comparable in appearance and indication reaction to those previously obtained with previously tested and approved lot of medium occurs for the specified temperature for a period of time within the range specified inoculating <= 100 cfu (at 30-35°C for 18-24 hours).
Cultural Response
| Organism | Inoculum (CFU) | Growth | Observed Lot Recovery value (CFU) | Colour of colony | Incubation temperature | |
|---|---|---|---|---|---|---|
| Escherichia coli ATCC 8739 (00012*) | 50-100 | luxuriant | 25-100 | >=50% | pink-red with bile precipitate | 18-24 hrs |
| ^Pseudomonas paraeruginosa ATCC 9027 (00026*) | 50-100 | luxuriant | 25-100 | >=50% | pink to red | 18-24 hrs |
Key :-(*) Corresponding WDCM numbers
^ Formerly known as Pseudomonas aeruginosa
Storage and Shelf Life
On receipt store between 15-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (6,7).
| Product Name | Violet Red Bile Glucose Agar |
|---|---|
| SKU | SMH581 |
| Customized Product Available | No |
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