Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Simmons Citrate Agar Slant
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SKU:
SL034
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Intended Use
Recommended for differentiating members of Enterobacteriaceae on the basis of citrate utilization.
Composition
| Ingredients | g/L |
|---|---|
| Magnesium sulphate | 0.200 |
| Ammonium dihydrogen phosphate | 1.000 |
| Dipotassium phosphate | 1.000 |
| Sodium citrate | 2.000 |
| Sodium chloride | 5.000 |
| Bromothymol blue | 0.080 |
| Agar | 15.000 |
| Final pH (at 25°C) | 6.8±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Streak the test inoculum aseptically into the slant and incubate at appropriate conditions. Incubate the slants at 35-37°C for 18-24 hours.
Principle And Interpretation
These media are used for the differentiation between Enterobacteriaceae and the members of aerogenes group on the basis of citrate utilization as sole carbon source. Initially the citrate medium was developed by Koser (1) containing ammonium salt as the only nitrogen source and citrate as the only carbon source for differentiating Escherichia coli and Enterobacter aerogenes by IMViC tests. Later on Simmons (2) modified Kosers formulation by adding agar and bromothymol blue (3). It is recommended by APHA (4). Ammonium dihydrogen phosphate and sodium citrate serve as the sole nitrogen and carbon source respectively. Microorganisms also use inorganic ammonium salts as their sole nitrogen source. Metabolism of these salts causes the medium to become alkaline, indicated by a change in colour of the pH indicator from green to blue. Bromothymol blue is the pH indicator. The medium should be freshly prepared because in dry conditions, changes in colour may appear even before inoculation, especially at the bottom of the slant.
Type of specimen
Isolated microorganism from clinical specimen and non clinical samples.
Specimen Collection and Handling
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (5,6,7). For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards (8). For clinical samples follow appropriate techniques for handling specimens as per established guidelines (9,10). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use. For professional use only. Read the label before opening the container. Wear protective gloves/ protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- The pH affects the performance of the medium and must be correctly monitored, hence appearance must be verified before inoculation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Sterile Simmons Citrate agar slant in glass tube.
Colour of medium: Forest green coloured slant
Quantity of medium: 8ml of medium in glass tube
pH: 6.60-7.00
Sterility Check: Passes release criteria
Cultural Response: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Citrate utilisation |
|---|---|---|---|
| # Klebsiella aerogenes ATCC 13048 (00175*) | 50-100 | good-luxuriant | positive reaction, blue colour |
| Escherichia coli ATCC 25922 (00013*) | >=104 | inhibited | |
| Salmonella Typhi ATCC 6539 | 50-100 | fair-good | negative reaction, green colour |
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | good-luxuriant | positive reaction, blue colour |
| Shigella dysenteriae ATCC 13313 | >=104 | inhibited | |
| Salmonella Choleraesuis ATCC 12011 | 50-100 | good-luxuriant | positive reaction, blue colour |
| Salmonella Enteritidis ATCC 13076 (00030*) | 50-100 | good-luxuriant | positive reaction, blue colour |
*: Corresponding WDCM numbers
#: Formerly known as Enterobacter aerogenes
Storage and Shelf Life
On receipt store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (9,10).
| Product Name | Simmons Citrate Agar Slant |
|---|---|
| SKU | SL034 |
| Customized Product Available | No |
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