Motility Indole Lysine Agar Butt

This slant is recommended for identification of members of Enterobacteriaceae on the basis of motility, lysine decarboxylase, lysine deaminase & indole production. recommended to be used along with Triple Sugar Iron Agar (TSI) (M021) and Urea Agar (M112) so as to enable presumptive identification of members of Enterobacteriaceae from faecal specimens. Growth of motile organisms extends out from the line of inoculation, while non-motile organisms grow only along the stab line. Bromocresol purple serves as the pH indicator. Motility, lysine deamination and lysine decarboxylation reactions are read before testing indole reaction, since addition of Kovac’s reagent causes the colour of the medium to change to yellow. Therefore positive lysine decarboxylase reaction could be misinterpreted as negative.